Cdc42 Antibody (B-8): sc-8401

Cdc42 Antibody (B-8) is a mouse monoclonal IgG3 kappa light chain antibody engineered to detect Cdc42, also known as Cell Division Control Protein 42 Homolog or G25K GTP-binding Protein. Anti-Cdc42 antibody (B-8) targets a precise epitope spanning amino acids 166-182 at the C-terminus of human Cdc42. Cdc42 is a pivotal member of the Rho family of GTPases within the larger Ras superfamily, playing a crucial role in regulating diverse cellular processes including cell morphology, migration, cell cycle progression, and cytoskeletal dynamics. These functions are fundamental to normal cellular operations and development, and dysregulation of Cdc42 has been linked to various pathological conditions, including cancer, neurodegenerative diseases, and developmental disorders, highlighting its significance as a therapeutic target and research focus. Cdc42 monoclonal antibody (B-8) exhibits broad species reactivity, effectively recognizing Cdc42 proteins from mouse, rat, human, Drosophila, Xenopus, as well as additional species such as equine, canine, bovine, porcine, and avian, making it a versatile tool for a wide range of experimental applications. Anti-Cdc42 antibody (B-8) is compatible with multiple techniques including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), facilitating comprehensive studies of Cdc42 expression and function. Available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, Cdc42 (B-8) antibody provides flexibility to meet diverse research needs. By leveraging Cdc42 monoclonal antibody (B-8), researchers can effectively investigate the signaling pathways and molecular mechanisms mediated by Cdc42, thereby advancing our understanding of cell growth, division, and the underlying causes of related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdc42 Antibody (B-8) References:

1. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product.  |  Hart, MJ., et al. 1991. Nature. 354: 311-4. PMID: 1956381
2. The cellular functions of small GTP-binding proteins.  |  Hall, A. 1990. Science. 249: 635-40. PMID: 2116664
3. Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42.  |  Munemitsu, S., et al. 1990. Mol Cell Biol. 10: 5977-82. PMID: 2122236
4. The GTPase superfamily: a conserved switch for diverse cell functions.  |  Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
5. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
6. Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.  |  Johnson, DI. and Pringle, JR. 1990. J Cell Biol. 111: 143-52. PMID: 2164028
7. CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae.  |  Adams, AE., et al. 1990. J Cell Biol. 111: 131-42. PMID: 2195038
8. Purification of the major GTP-binding proteins from human placental membranes.  |  Evans, T., et al. 1986. J Biol Chem. 261: 7052-9. PMID: 3084494

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc42 Antibody (B-8) has 183 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc42 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc42 Antibody (B-8) Specifications

Alternate Product Name: Cdc42

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 166-182

Epitope Target: Cdc42

Epitope Species: human

Product Reactivity/Detection: Cdc42

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: Drosophila, Xenopus

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 183 citations in various scientific publications

Available Conjugates/Forms for Cdc42 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Cdc42 antibody is Cdc42 Antibody (B-9): sc-390210.