Cdc4 Antibody (3D1) recommended for detection of Cdc4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cdc4 Antibody (3D1): sc-293423

Cdc4 Antibody (3D1) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-Cdc4 Antibody (3D1) is a mouse monoclonal IgG2a (kappa light chain) Cdc4 antibody provided at 100 µg/ml
    • raised against amino acids 599-707 representing partial length Cdc4 of human origin
    • recommended for detection of Cdc4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc4 (3D1): sc-293423.
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Can sc-293423: Cdc4 (3D1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-293423: Cdc4 (3D1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from The band is a lillte weak 1:100 diluted for wb of rat protein,the band is a little weak.
Date published: 2017-12-31
Rated 4 out of 5 by from good The concentration we used for WB is 1:200, and it worked well.
Date published: 2017-07-06
Rated 4 out of 5 by from positive staining IHC staining mouse lung tissue at dilution 1/200, I got positive staining, the result is perfect.
Date published: 2016-12-21
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