Cdc4 Antibody (A-2): sc-518091
- Cdc4 Antibody (A-2) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 351-375 within an internal region of Cdc4 of human origin
- Anti-Cdc4 Antibody (A-2) is recommended for detection of Cdc4 of and human origin by WB, IP, IF and ELISA
- Anti-Cdc4 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Cdc4 Antibody (A-2) for WB applications. This reagent is now offered in a bundle with Cdc4 Antibody (A-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Cdc4 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc4 (A-2) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc4 plays a crucial role in cell cycle regulation by targeting specific proteins for degradation, ensuring proper cell division and preventing uncontrolled cell proliferation. Mutations in Cdc4 have been linked to ovarian and breast cancer development, highlighting Cdc4′s potential as a biomarker for tumor progression and therapeutic target. As a member of the F-box protein family, Cdc4 contains a 40 amino acid F-box motif and forms part of the SCF ubiquitin ligase complex, essential for phosphorylation-dependent ubiquitination. Cdc4 also contains seven tandem WD40 repeats that facilitate protein-protein interactions, emphasizing Cdc4′s importance in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc4 Antibody (A-2) References:
- Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4. | Jäger, S., et al. 2001. EMBO J. 20: 4423-31. PMID: 11500370
- Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. | Perkins, G., et al. 2001. EMBO J. 20: 4836-45. PMID: 11532947
- CDC4 gene expression as potential biomarker for targeted therapy in prostate cancer. | Koh, MS., et al. 2006. Cancer Biol Ther. 5: 78-83. PMID: 16357515
- A refined two-hybrid system reveals that SCF(Cdc4)-dependent degradation of Swi5 contributes to the regulatory mechanism of S-phase entry. | Kishi, T., et al. 2008. Proc Natl Acad Sci U S A. 105: 14497-502. PMID: 18787112
- Somatic mutations of the CDC4 (FBXW7) gene in hereditary colorectal tumors. | Miyaki, M., et al. 2009. Oncology. 76: 430-4. PMID: 19420964
- Sirtuin 7 plays an oncogenic role in human osteosarcoma via downregulating CDC4 expression. | Wei, W., et al. 2017. Am J Cancer Res. 7: 1788-1803. PMID: 28979804
- Regulatory crosstalk between KLF5, miR-29a and Fbw7/CDC4 cooperatively promotes atherosclerotic development. | Zheng, B., et al. 2018. Biochim Biophys Acta Mol Basis Dis. 1864: 374-386. PMID: 29074464
- Scutellarein inhibits the development of colon cancer via CDC4‑mediated RAGE ubiquitination. | Li, Y., et al. 2020. Int J Mol Med. 45: 1059-1072. PMID: 32124957
- SCFCdc4 ubiquitin ligase regulates synaptonemal complex formation during meiosis. | Zhu, Z., et al. 2021. Life Sci Alliance. 4: PMID: 33293336
- Cdc4 phospho-degrons allow differential regulation of Ame1CENP-U protein stability across the cell cycle. | Böhm, M., et al. 2021. Elife. 10: PMID: 34308839
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc4 Antibody (A-2) | sc-518091 | 200 µg/ml | $322.00 | |||
Cdc4 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-525361 | 200 µg Ab, 40 µg BP | $361.00 | |||
Cdc4 Antibody (A-2) AC | sc-518091 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc4 Antibody (A-2) HRP | sc-518091 HRP | 200 µg/ml | $322.00 | |||
Cdc4 Antibody (A-2) FITC | sc-518091 FITC | 200 µg/ml | $336.00 | |||
Cdc4 Antibody (A-2) PE | sc-518091 PE | 200 µg/ml | $349.00 | |||
Cdc4 Antibody (A-2) Alexa Fluor® 488 | sc-518091 AF488 | 200 µg/ml | $364.00 | |||
Cdc4 Antibody (A-2) Alexa Fluor® 546 | sc-518091 AF546 | 200 µg/ml | $364.00 | |||
Cdc4 Antibody (A-2) Alexa Fluor® 594 | sc-518091 AF594 | 200 µg/ml | $364.00 | |||
Cdc4 Antibody (A-2) Alexa Fluor® 647 | sc-518091 AF647 | 200 µg/ml | $364.00 | |||
Cdc4 Antibody (A-2) Alexa Fluor® 680 | sc-518091 AF680 | 200 µg/ml | $364.00 | |||
Cdc4 Antibody (A-2) Alexa Fluor® 790 | sc-518091 AF790 | 200 µg/ml | $364.00 |