Cdc37 Antibody (E-4): sc-13129

Cdc37 Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cdc37 of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Cdc37 (E-4) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc37 plays a crucial role in cell cycle regulation by facilitating the formation of cyclin-dependent kinase 4 (Cdk4) and cyclin D1 complex, essential for G1 to S phase transition. Cdc37 forms a stable complex with heat shock protein 90 (Hsp90), important for stability and function of various client proteins involved in cell cycle regulation. Proper functioning of Cdc37 and associated protein interactions are critical for maintaining cellular homeostasis and preventing uncontrolled cell proliferation, which can lead to cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdc37 Antibody (E-4) References:

1. Fused kinase is stabilized by Cdc37/Hsp90 and enhances Gli protein levels.  |  Kise, Y., et al. 2006. Biochem Biophys Res Commun. 351: 78-84. PMID: 17054904
2. Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation.  |  Mandal, AK., et al. 2007. J Cell Biol. 176: 319-28. PMID: 17242065
3. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
4. Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae.  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
5. The Therapeutic Potential of Targeting Hsp90-Cdc37 Interactions in Several Diseases.  |  Zhang, X., et al. 2022. Curr Drug Targets. 23: 1023-1038. PMID: 35400341
6. Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation.  |  García-Alonso, S., et al. 2022. Mol Cell. 82: 3438-3452.e8. PMID: 36055235
7. Predictive value of CDC37 gene expression for targeted therapy in metastatic colorectal cancer.  |  Arai, H., et al. 2024. Eur J Cancer. 201: 113914. PMID: 38359495
8. A new twist to the cell cycle.  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
9. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
10. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
11. Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
12. A human protein related to yeast Cdc6p.  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc37 Antibody (E-4) has 39 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc37 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc37 Antibody (E-4) Specifications

Alternate Product Name: Cdc37

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 108-378

Epitope Target: Cdc37

Epitope Species: human

Product Reactivity/Detection: Cdc37

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 39 citations in various scientific publications

Available Conjugates/Forms for Cdc37 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine