Cdc37 Antibody (E-4): sc-13129
- Cdc37 Antibody (E-4) is a mouse monoclonal IgG2b κ Cdc37 antibody, cited in 40 publications, provided at 200 µg/ml
- raised against amino acids 108-378 of Cdc37 of human origin
- Cdc37 Antibody (E-4) is recommended for detection of Cdc37 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Cdc37 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to TRITC for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc37 Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc37 Antibody (E-4) (see ordering information below).
Cdc37 Antibody (E-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cdc37 of mouse, rat, and human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Cdc37 (E-4) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc37 plays a crucial role in cell cycle regulation by facilitating the formation of cyclin-dependent kinase 4 (Cdk4) and cyclin D1 complex, essential for G1 to S phase transition. Cdc37 forms a stable complex with heat shock protein 90 (Hsp90), important for stability and function of various client proteins involved in cell cycle regulation. Proper functioning of Cdc37 and associated protein interactions are critical for maintaining cellular homeostasis and preventing uncontrolled cell proliferation, which can lead to cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc37 Antibody (E-4) References:
- Fused kinase is stabilized by Cdc37/Hsp90 and enhances Gli protein levels. | Kise, Y., et al. 2006. Biochem Biophys Res Commun. 351: 78-84. PMID: 17054904
- Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation. | Mandal, AK., et al. 2007. J Cell Biol. 176: 319-28. PMID: 17242065
- cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2. | Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
- Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae. | Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
- The Therapeutic Potential of Targeting Hsp90-Cdc37 Interactions in Several Diseases. | Zhang, X., et al. 2022. Curr Drug Targets. 23: 1023-1038. PMID: 35400341
- Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. | García-Alonso, S., et al. 2022. Mol Cell. 82: 3438-3452.e8. PMID: 36055235
- Predictive value of CDC37 gene expression for targeted therapy in metastatic colorectal cancer. | Arai, H., et al. 2024. Eur J Cancer. 201: 113914. PMID: 38359495
- A new twist to the cell cycle. | Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
- A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. | King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
- Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. | Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
- Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4. | Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
- A human protein related to yeast Cdc6p. | Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc37 Antibody (E-4) | sc-13129 | 200 µg/ml | $322.00 | |||
Cdc37 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-528265 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cdc37 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-520600 | 200 µg Ab, 40 µg BP | $361.00 | |||
Cdc37 Antibody (E-4): m-IgG2b BP-HRP Bundle | sc-550016 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cdc37 Antibody (E-4) AC | sc-13129 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc37 Antibody (E-4) HRP | sc-13129 HRP | 200 µg/ml | $322.00 | |||
Cdc37 Antibody (E-4) FITC | sc-13129 FITC | 200 µg/ml | $336.00 | |||
Cdc37 Antibody (E-4) PE | sc-13129 PE | 200 µg/ml | $349.00 | |||
Cdc37 Antibody (E-4) Alexa Fluor® 488 | sc-13129 AF488 | 200 µg/ml | $364.00 | |||
Cdc37 Antibody (E-4) Alexa Fluor® 546 | sc-13129 AF546 | 200 µg/ml | $364.00 | |||
Cdc37 Antibody (E-4) Alexa Fluor® 594 | sc-13129 AF594 | 200 µg/ml | $364.00 | |||
Cdc37 Antibody (E-4) Alexa Fluor® 647 | sc-13129 AF647 | 200 µg/ml | $364.00 | |||
Cdc37 Antibody (E-4) Alexa Fluor® 680 | sc-13129 AF680 | 200 µg/ml | $364.00 | |||
Cdc37 Antibody (E-4) Alexa Fluor® 790 | sc-13129 AF790 | 200 µg/ml | $364.00 | |||
Cdc37 Antibody (E-4) TRITC | sc-13129 TRITC | 200 µg/ml | $326.00 |