Cdc27 Antibody (AF3.1): sc-9972

Cdc27 Antibody (AF3.1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cdc27 protein of mouse, rat, human, and Xenopus laevis origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Cdc27 monoclonal antibody (AF3.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc27 plays a crucial role in the regulation of the cell cycle, particularly as a component of the anaphase-promoting complex (APC), which is essential for the timely degradation of cyclins and other regulatory proteins. This degradation is vital for the transition between different phases of the cell cycle, ensuring that cells divide correctly and at the appropriate time. By facilitating the ubiquitination of cyclin B, Cdc27 helps to trigger the metaphase-anaphase transition, thereby preventing errors in chromosome segregation that could lead to aneuploidy or other cell cycle-related disorders. Proper functioning of Cdc27 and the APC is therefore critical for maintaining genomic stability and preventing tumorigenesis, making Cdc27 (AF3.1) antibody an important tool for researchers studying cell cycle regulation and its implications in cancer biology.

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Cdc27 Antibody (AF3.1) References:

1. Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase.  |  Kumar, P. and Wang, CC. 2005. J Biol Chem. 280: 31783-91. PMID: 15994309
2. RNA interference knockdown of hU2AF35 impairs cell cycle progression and modulates alternative splicing of Cdc25 transcripts.  |  Pacheco, TR., et al. 2006. Mol Biol Cell. 17: 4187-99. PMID: 16855028
3. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
4. Phosphorylation of the anaphase-promoting complex/Cdc27 is involved in TGF-beta signaling.  |  Zhang, L., et al. 2011. J Biol Chem. 286: 10041-50. PMID: 21209074
5. Mitotic transmission of artificial chromosomes in cdc mutants of the yeast, Saccharomyces cerevisiae.  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
6. Identification of a novel protein interaction between Elmo1 and Cdc27.  |  Lee, J., et al. 2016. Biochem Biophys Res Commun. 471: 497-502. PMID: 26882976
7. CDC27 Induces Metastasis and Invasion in Colorectal Cancer via the Promotion of Epithelial-To-Mesenchymal Transition.  |  Qiu, L., et al. 2017. J Cancer. 8: 2626-2635. PMID: 28900500
8. A new twist to the cell cycle.  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
9. A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
10. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
11. Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4.  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
12. A human protein related to yeast Cdc6p.  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc27 Antibody (AF3.1) has 73 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc27 Antibody (AF3.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc27 Antibody (AF3.1) Specifications

Alternate Product Name: Cdc27

Product Clone Name: AF3.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 814-823

Epitope Target: Cdc27

Epitope Species: human

Product Reactivity/Detection: Cdc27

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 73 citations in various scientific publications

Available Conjugates/Forms for Cdc27 Antibody (AF3.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin