caspase-8 p10 Antibody (C502S): sc-56071

Caspase-8 p10 Antibody (C502S) is a mouse monoclonal IgG1 antibody engineered against a synthetic peptide corresponding to the N-terminus of the human caspase-8 p10 subunit. Anti-caspase-8 p10 antibody (C502S) effectively recognizes human caspase-8 in western blotting (WB) and immunoprecipitation (IP) assays without cross-reacting with its precursor forms, ensuring precise detection of the active enzyme. Caspase-8 is a pivotal initiator caspase located primarily in the cytoplasm, where caspase-8 plays a critical role in the extrinsic apoptosis pathway. Upon activation of death receptors such as TNFRSF1A and TNFRSF6/FAS, caspase-8 is recruited to the death-inducing signaling complex (DISC) through interaction with the adaptor molecule FADD. This localization to the DISC facilitates rapid activation of caspase-8, which subsequently cleaves and activates downstream effector caspases, leading to the execution of apoptosis. Cytoplasmic presence of caspase-8 is essential for swift response to apoptotic signals, thereby maintaining cellular homeostasis and immune function. Caspase-8 is ubiquitously expressed with particularly high levels in peripheral blood leukocytes, thymus, liver, and spleen. Mutations or deficiencies in the CASP8 gene, which encodes caspase-8, can result in CASP8 deficiency disorder (CASP8D), characterized by splenomegaly and impaired apoptosis of peripheral blood leukocytes, ultimately leading to immunodeficiency due to defects in T lymphocyte, NK cell, and B lymphocyte activation. Caspase-8 p10 (C502S) monoclonal antibody is an essential tool for researchers studying apoptosis, immune regulation, and related pathologies, providing reliable detection of active caspase-8 within critical cellular context.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

caspase-8 p10 Antibody (C502S) References:

1. Caspase-8 as a regulator of tumor cell motility.  |  Graf, RP., et al. 2014. Curr Mol Med. 14: 246-54. PMID: 24467204
2. Caspase-8: A Novel Target to Overcome Resistance to Chemotherapy in Glioblastoma.  |  Fianco, G., et al. 2018. Int J Mol Sci. 19: PMID: 30501030
3. Activity of caspase-8 determines plasticity between cell death pathways.  |  Newton, K., et al. 2019. Nature. 575: 679-682. PMID: 31723262
4. Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis.  |  Tummers, B., et al. 2020. Immunity. 52: 994-1006.e8. PMID: 32428502
5. The role of caspase-8 in the tumor microenvironment of ovarian cancer.  |  Kostova, I., et al. 2021. Cancer Metastasis Rev. 40: 303-318. PMID: 33026575
6. Blood caspase-8 concentrations and mortality among septic patients.  |  Lorente, L., et al. 2022. Med Intensiva (Engl Ed). 46: 8-13. PMID: 34991877
7. Caspase-8 and FADD prevent spontaneous ZBP1 expression and necroptosis.  |  Rodriguez, DA., et al. 2022. Proc Natl Acad Sci U S A. 119: e2207240119. PMID: 36191211
8. Caspase-8 is required for HSV-1-induced apoptosis and promotes effective viral particle release via autophagy inhibition.  |  Marino-Merlo, F., et al. 2023. Cell Death Differ. 30: 885-896. PMID: 36418547
9. Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activation.  |  Lentini, G., et al. 2023. Cell Rep Med. 4: 101098. PMID: 37390829
10. Dual Role of Caspase 8 in Adipocyte Apoptosis and Metabolic Inflammation.  |  Luk, CT., et al. 2023. Diabetes. 72: 1751-1765. PMID: 37699387

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.