caspase-8 p10 Antibody (C502S) recommended for detection of p10 subunit of caspase-8 of human origin by WB and IP

caspase-8 p10 Antibody (C502S): sc-56071

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Datasheets

    • caspase-8 p10 Antibody (C502S) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against a synthetic peptide corresponding to the N-terminus of caspase-8 p10 of Caspase 8 of human origin
    • recommended for detection of p10 subunit of caspase-8 of human origin by WB and IP; non cross-reactive with precursor caspase-8
    • See caspase-8 (8CSP03): sc-56070 for caspase-8 p10 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using caspase-8 p10 (C502S): sc-56071 antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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