caspase-7 p20 Antibody (B-5): sc-28295
- caspase-7 p20 Antibody (B-5) is a mouse monoclonal IgG1 κ, cited in 16 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 15-50 near the N-terminus of caspase-7 p20 of human origin
- Anti-caspase-7 p20 Antibody (B-5) is recommended for detection of p20 subunit and precursor of caspase-7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
- Anti-caspase-7 p20 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for caspase-7 p20 Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-7 p20 Antibody (B-5) (see ordering information below).
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caspase-7 p20 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the p20 subunit and precursor of caspase-7 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-caspase-7 antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-7 is a crucial member of the Ced-3/caspase-1 family of cysteine proteases, which are integral to the apoptotic process, facilitating programmed cell death by cleaving specific target proteins that regulate cellular longevity. The p20 subunit of caspase-7 plays a role in the cleavage of poly(ADP-ribose) polymerase (PARP), a nuclear protein involved in DNA repair and cellular stress responses. The ability of caspase-7 to cleave PARP into an apoptotic fragment underscores its significance in apoptosis, as this process is vital for maintaining cellular homeostasis and preventing the propagation of damaged cells. Caspase-7 is activated by upstream caspases, such as caspase-10, in response to apoptotic signals, further highlighting its role in the intricate network of cellular signaling pathways that govern cell death and survival.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
caspase-7 p20 Antibody (B-5) References:
- Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis. Evidence for involvement of caspase-7. | Germain, M., et al. 1999. J Biol Chem. 274: 28379-84. PMID: 10497198
- Yeast two-hybrid screening using constitutive-active caspase-7 as bait in the identification of PA28gamma as an effector caspase substrate. | Araya, R., et al. 2002. Cell Death Differ. 9: 322-8. PMID: 11859414
- Inactivating mutations of CASPASE-7 gene in human cancers. | Soung, YH., et al. 2003. Oncogene. 22: 8048-52. PMID: 12970753
- Caspase-7 gene disruption reveals an involvement of the enzyme during the early stages of apoptosis. | Korfali, N., et al. 2004. J Biol Chem. 279: 1030-9. PMID: 14583630
- Chromosomal localization of the human genes, CPP32, Mch2, Mch3, and Ich-1, involved in cellular apoptosis. | Tiso, N., et al. 1996. Biochem Biophys Res Commun. 225: 983-9. PMID: 8780721
- Caspases: the executioners of apoptosis. | Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844
- Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver. | Chandler, JM., et al. 1998. J Biol Chem. 273: 10815-8. PMID: 9556551
- Signaling pathway activated during apoptosis of the prostate cancer cell line LNCaP: overexpression of caspase-7 as a new gene therapy strategy for prostate cancer. | Marcelli, M., et al. 1999. Cancer Res. 59: 382-90. PMID: 9927051
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-7 p20 Antibody (B-5) | sc-28295 | 200 µg/ml | $322.00 | |||
caspase-7 p20 Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-526655 | 200 µg Ab; 10 µg BP | $361.00 | |||
caspase-7 p20 Antibody (B-5): m-IgG1 BP-HRP Bundle | sc-532028 | 200 µg Ab; 20 µg BP | $361.00 | |||
caspase-7 p20 Antibody (B-5) AC | sc-28295 AC | 500 µg/ml, 25% agarose | $424.00 | |||
caspase-7 p20 Antibody (B-5) HRP | sc-28295 HRP | 200 µg/ml | $322.00 | |||
caspase-7 p20 Antibody (B-5) FITC | sc-28295 FITC | 200 µg/ml | $336.00 | |||
caspase-7 p20 Antibody (B-5) PE | sc-28295 PE | 200 µg/ml | $349.00 | |||
caspase-7 p20 Antibody (B-5) Alexa Fluor® 488 | sc-28295 AF488 | 200 µg/ml | $364.00 | |||
caspase-7 p20 Antibody (B-5) Alexa Fluor® 546 | sc-28295 AF546 | 200 µg/ml | $364.00 | |||
caspase-7 p20 Antibody (B-5) Alexa Fluor® 594 | sc-28295 AF594 | 200 µg/ml | $364.00 | |||
caspase-7 p20 Antibody (B-5) Alexa Fluor® 647 | sc-28295 AF647 | 200 µg/ml | $364.00 | |||
caspase-7 p20 Antibody (B-5) Alexa Fluor® 680 | sc-28295 AF680 | 200 µg/ml | $364.00 | |||
caspase-7 p20 Antibody (B-5) Alexa Fluor® 790 | sc-28295 AF790 | 200 µg/ml | $364.00 | |||
caspase-7 p20 (B-5) Neutralizing Peptide | sc-28295 P | 100 µg/0.5 ml | $69.00 |