caspase-3 p17 Antibody (D-12): sc-373730

Caspase-3 p17 Antibody (D-12) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect the p17 subunit of caspase-3, also known as apopain, SCA-1, Yama, and CPP32. Anti-caspase-3 p17 antibody (D-12) targets an epitope between amino acids 50-86 within an internal region of human caspase-3 p17, ensuring precise recognition of both the p17 subunit and the full-length procaspase-3 across mouse, rat, and human species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) assays. Caspase-3 is a crucial aspartate-specific cysteine protease within the ICE subfamily, playing a fundamental role in the execution phase of apoptosis, the programmed cell death process essential for development, tissue homeostasis, and the removal of damaged or unwanted cells. Structurally, caspase-3 is synthesized as an inactive proenzyme, procaspase-3, which undergoes proteolytic cleavage to form the active enzyme composed of two p17 and two p11 subunits, creating a heterotetramer necessary for enzymatic activity. Caspase-3 interacts with several key proteins, including PARP (poly ADP ribose polymerase), which caspase-3 cleaves to facilitate the disassembly of cellular components during apoptosis, and activates downstream caspases such as caspase-6, -7, and -9, thereby amplifying the apoptotic signal. Additionally, caspase-3 interacts with SREBPs (sterol regulatory element-binding proteins) by cleaving them from the endoplasmic reticulum membrane, a process distinct from their normal activation pathway. These interactions are vital for regulating the apoptotic pathways, ensuring the orderly execution of cell death, and maintaining cellular integrity. Caspase-3 is predominantly localized in the cytoplasm, allowing rapid response to apoptotic signals and interaction with various substrates to drive the cell death process. Caspase-3 is highly expressed in vital organs including the lung, spleen, heart, liver, kidney, and immune cells, underscoring a pivotal role in numerous physiological processes and disease mechanisms such as cancer, neurodegenerative disorders, and immune responses.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-3 p17 Antibody (D-12) has 45 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-3 p17 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-3 p17 Antibody (D-12) Specifications

Alternate Product Name: caspase-3 p17

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 50-86

Epitope Target: caspase-3 p17

Epitope Species: human

Product Reactivity/Detection: caspase-3 p17 and full length procaspase-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 45 citations in various scientific publications

Available Conjugates/Forms for caspase-3 p17 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred caspase-3 antibody is caspase-3 Antibody (E-8): sc-7272.