caspase-3 Antibody (31A1067): sc-56053

caspase-3 Antibody (31A1067) is a mouse monoclonal IgG1 antibody designed to detect both active caspase-3 and its inactive precursor, procaspase-3, across multiple species including mouse, rat, and human. Caspase-3, also known as apopain, SCA-1, Yama, and CPP32, is a crucial aspartate-specific cysteine protease within the ICE subfamily of caspases. Caspase-3 plays a pivotal role in the execution phase of apoptosis, the programmed cell death process essential for maintaining cellular homeostasis and eliminating damaged or unwanted cells. Upon activation, caspase-3 cleaves key substrates like PARP, leading to the dismantling of cellular components necessary for apoptosis. Additionally, caspase-3 is involved in activating other caspases, including caspase-6, -7, and -9, thereby amplifying the apoptotic signal. The human caspase-3 gene encodes a cytoplasmic protein abundantly expressed in vital organs such as the lung, spleen, heart, liver, and kidney, as well as in immune cells. Understanding and detecting caspase-3 activity is essential for research in fields like cancer, neurodegeneration, and immune response, where apoptosis plays a significant role. Caspase-3 (31A1067) monoclonal antibody is versatile and suitable for various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA).

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-3 Antibody (31A1067) has 297 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-3 Antibody (31A1067) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-3 Antibody (31A1067) Specifications

Alternate Product Name: caspase-3

Product Clone Name: 31A1067

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: caspase-3

Epitope Species: human

Product Reactivity/Detection: caspase-3 and full length procaspase-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 297 citations in various scientific publications

Available Conjugates/Forms for caspase-3 Antibody (31A1067): N/A

The preferred caspase-3 antibody is caspase-3 Antibody (E-8): sc-7272.