caspase-14 Antibody (D-10): sc-48336

caspase-14 Antibody (D-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects caspase-14 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-caspase-14 antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-14, also known as MICE (mini-ICE), plays a crucial role in apoptotic processes, particularly during embryonic development, where expression levels are high. Caspase-14 is unique among the caspase family due to specific processing and activation pathways; caspase-8 and caspase-10 can cleave caspase-14, but other caspases cannot, highlighting specialized function in apoptosis. Caspase-14 presence in embryonic tissues suggests importance in regulating cell death during development, ensuring proper tissue formation and homeostasis. Caspase-14 is largely absent in adult tissues, indicating a potential shift in role as organisms mature. Understanding caspase-14 function and regulation is vital for elucidating contributions to both normal physiological processes and pathological conditions, such as cancer and neurodegenerative diseases, where apoptosis plays a significant role.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

caspase-14 Antibody (D-10) References:

1. Identification of a new caspase homologue: caspase-14.  |  Van de Craen, M., et al. 1998. Cell Death Differ. 5: 838-46. PMID: 10203698
2. Caspase-14 and epidermis maturation.  |  Nicotera, P. and Melino, G. 2007. Nat Cell Biol. 9: 621-2. PMID: 17541415
3. Caspase-14 suppresses GCM1 acetylation and inhibits placental cell differentiation.  |  Wu, YH., et al. 2013. FASEB J. 27: 2818-28. PMID: 23580611
4. Caspase-14 expression impairs retinal pigment epithelium barrier function: potential role in diabetic macular edema.  |  Beasley, S., et al. 2014. Biomed Res Int. 2014: 417986. PMID: 25121097
5. Caspase-14-From Biomolecular Basics to Clinical Approach. A Review of Available Data.  |  Markiewicz, A., et al. 2021. Int J Mol Sci. 22: PMID: 34070382
6. mRNA expression of caspase 14 in skin epithelial malignancies.  |  Markiewicz, A., et al. 2023. Postepy Dermatol Alergol. 40: 315-320. PMID: 37312914
7. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
8. Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death.  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
9. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
10. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
11. Caspase-14 is a novel developmentally regulated protease.  |  Hu, S., et al. 1998. J Biol Chem. 273: 29648-53. PMID: 9792675
12. Identification and characterization of murine caspase-14, a new member of the caspase family.  |  Ahmad, M., et al. 1998. Cancer Res. 58: 5201-5. PMID: 9823333

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-14 Antibody (D-10) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-14 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-14 Antibody (D-10) Specifications

Alternate Product Name: caspase-14

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 24-122

Epitope Target: caspase-14

Epitope Species: human

Product Reactivity/Detection: caspase-14

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for caspase-14 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin