caspase-1 Antibody (14F468): sc-56036

caspase-1 Antibody (14F468) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect caspase-1, also known as IL-1 converting enzyme (ICE), in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. caspase-1 (14F468) antibody targets the amino acid region 371-390 of human caspase-1, enabling recognition of both full-length and cleaved forms across multiple species including mouse, rat, human, and porcine. Caspase-1 plays a pivotal role in the inflammatory response by mediating the maturation of pro-inflammatory cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) through the proteolytic cleavage of their inactive precursors. Additionally, caspase-1 is involved in the induction of apoptosis, contributing to the structural dismantling of cells during programmed cell death. The active form of caspase-1 is a tetramer composed of p20 and p10 subunits, essential for enzymatic activity. The human CASP1 gene is located on chromosome 2q14 and is expressed in key tissues such as the liver, heart, skeletal muscle, kidney, and testis. Dysregulation of caspase-1 has been implicated in various pathological conditions including inflammation, septic shock, impaired wound healing, and certain leukemias, underscoring its importance in both normal physiology and disease states. anti-caspase-1 antibody (14F468) provides reliable and specific detection of caspase-1, facilitating research into diverse biological functions and role in disease mechanisms.

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caspase-1 Antibody (14F468) References:

1. Roles of caspases in apoptosis, development, and cytokine maturation revealed by homozygous gene deficiencies.  |  Wang, J. and Lenardo, MJ. 2000. J Cell Sci. 113 (Pt 5): 753-7. PMID: 10671365
2. The prodomain of caspase-1 enhances Fas-mediated apoptosis through facilitation of caspase-8 activation.  |  Tatsuta, T., et al. 2000. J Biol Chem. 275: 14248-54. PMID: 10799503
3. Caspase pathways, neuronal apoptosis, and CNS injury.  |  Eldadah, BA. and Faden, AI. 2000. J Neurotrauma. 17: 811-29. PMID: 11063050
4. Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells.  |  Ishioka, S., et al. 2006. Int J Med Sci. 4: 36-44. PMID: 17299580
5. NF-kappaB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1.  |  Miggin, SM., et al. 2007. Proc Natl Acad Sci U S A. 104: 3372-7. PMID: 17360653
6. Anthrax lethal toxin kills macrophages in a strain-specific manner by apoptosis or caspase-1-mediated necrosis.  |  Muehlbauer, SM., et al. 2007. Cell Cycle. 6: 758-66. PMID: 17374996
7. Targeting caspase-1 in sepsis: a novel approach to an old problem.  |  Matute-Bello, G. 2007. Intensive Care Med. 33: 755-757. PMID: 17384934
8. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity.  |  Boucher, D., et al. 2018. J Exp Med. 215: 827-840. PMID: 29432122
9. Caspase-1 as a regulatory molecule of lipid metabolism.  |  Molla, MD., et al. 2020. Lipids Health Dis. 19: 34. PMID: 32143623
10. The intricate biophysical puzzle of caspase-1 activation.  |  Makoni, NJ. and Nichols, MR. 2021. Arch Biochem Biophys. 699: 108753. PMID: 33453207
11. Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathway.  |  Wang, F., et al. 2022. Free Radic Biol Med. 182: 171-181. PMID: 35219847
12. Interleukin-1 beta converting enzyme requires oligomerization for activity of processed forms in vivo.  |  Gu, Y., et al. 1995. EMBO J. 14: 1923-31. PMID: 7743999

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-1 Antibody (14F468) has 364 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-1 Antibody (14F468) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-1 Antibody (14F468) Specifications

Alternate Product Name: caspase-1

Product Clone Name: 14F468

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 371-390

Epitope Target: caspase-1

Epitope Species: human

Product Reactivity/Detection: full-length caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the caspase-1 protein

Species Reactivity/Detection: mouse, rat, human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide

Research Citations: 364 citations in various scientific publications

Available Conjugates/Forms for caspase-1 Antibody (14F468): N/A

The preferred caspase-1 p20 antibody is caspase-1 p20 Antibody (D-4): sc-398715.