caspase-1 p20 Antibody (D-4): sc-398715

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Datasheets

- caspase-1 p20 Antibody (D-4) is a mouse monoclonal IgG_2b κ, cited in 88 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 259-294 at the C-terminus of caspase-1 p20 of mouse origin
- Anti-caspase-1 p20 Antibody (D-4) is recommended for detection of caspase-1 precursor and p20 subunit of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-caspase-1 p20 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-1 p20 (D-4): sc-398715.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for caspase-1 p20 Antibody (D-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-1 p20 Antibody (D-4) (see ordering information below).

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caspase-1 p20 Antibody (D-4) - Western Blotting - Image 308131

caspase-1 p20 Antibody (D-4) - Immunohistochemistry - Image 348064

caspase-1 p20 Antibody (D-4) - Western Blotting - Image 364938

caspase-1 p20 Antibody (D-4) - Immunohistochemistry - Image 348063

caspase-1 p20 Antibody (D-4) - Western Blotting - Image 379630

caspase-1 p20 Antibody (D-4) - Immunohistochemistry - Image 348062

caspase-1 p20 Antibody (D-4) - Western Blotting - Image 361604

caspase-1 p20 Antibody (D-4) - Western Blotting - Image 393440

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caspase-1 p20 Antibody (D-4) is an IgG2b κ mouse monoclonal caspase-1 p20 antibody suitable for the detection of caspase-1 precursor and p20 subunit of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA. caspase-1 p20 Antibody (D-4) is available as the non-conjugated anti-caspase-1 p20 antibody form, as well as multiple conjugated forms of anti-caspase-1 p20 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1 beta and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. The prodomain of caspase-1 (also known as Pro-C1) represents the amino acid terminal portion of the caspase-1 precursor. Pro-C1 is produced as a residual component after proteolytic cleavage of the precursor generates the functional caspase-1 subunits known as the p20 and p10 subunits. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Mouse	Casp1	Entrez Gene ID: 12362	9 A1	NM_009807	UniProt ID: P29452	N/A

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Protocols Online

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-1 p20 Antibody (D-4) has 88 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-1 p20 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-1 p20 Antibody (D-4) Specifications

Alternate Product Name

caspase-1 p20

Product Clone Name

D-4

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

259-294

Epitope Target

caspase-1 p20

Epitope Species

mouse

Product Reactivity/Detection

caspase-1 precursor and p20 subunit

Species Reactivity/Detection

mouse, rat

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

88 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase P: Blocking Peptide PE: Phycoerythrin

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-caspase-1 p20 Antibody (D-4) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

The preferred caspase-1 antibody is caspase-1 Antibody (14F468): sc-56036.

What is the expected molecular weight of the protein detected by this antibody, caspase-1 p20 (D-4): sc-398715?

Asked by: TinTin

This antibody, caspase-1 p20: sc-398715, is recommended for the detection of caspase-1 precursor and p20 subunit. The expected molecular weights of these caspase forms are ~45kDa and ~20kDa, respectively, however detection of the p20 subunit can be difficult to observe by Western blot, so it is more common to only see the precursor band.

Answered by: Technical Support 12

Date published: 2017-01-24

What application is the blocking peptide sc-398715 P appropriate for?

Asked by: jenni

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Service 11

Date published: 2019-05-25

Rated 5 out of 5 by 冠峰生物 from 条带清晰，背景底我对这个抗体感到非常满意，价格优惠，希望santa cruz公司推出更多优秀的抗体，为全球科学家提供更多有价值的产品，做出更多的贡献。

Date published: 2021-12-22

Rated 5 out of 5 by wuchan_3725 from 功能出色同事订购过此产品caspase-1 p20抗体 (D-4)，使用效果不错，条带清晰，我希望可以获取小样试用，将会非常高兴。

Date published: 2018-05-11

Rated 5 out of 5 by qin ziying01 from IHC Used it for IHC got very perfect staining for mouse colon section.1:150 very good concetration.

Date published: 2018-01-27

Rated 1 out of 5 by Phyumt from Caspase-1 p20 antibody I received a sample size to test for active caspase-1 in the medium of BMDCs stimulated in various conditions. I wasn't able to detect any bands at all. :(

Date published: 2018-01-06

Rated 5 out of 5 by oobianom from Good! Caspase 1 p-20(d-4) protein: from mouse liver extracted with RIPA Loading 20ug/well

Date published: 2017-12-12

Rated 5 out of 5 by TINGEr from excellent quality A good staining was obtained in rat colon tissue in IHC(1:100 dilution) and very good antibodies were obtained.

Date published: 2017-10-26

Rated 5 out of 5 by MoonWalker from 功能很好上次申请的小样试用装非常好用，它不仅能曝出全长的caspase 1蛋白条带，还能曝出caspase-1 p20条带。但是就是还有些扎蛋白条带。不过都不影响条带。

Date published: 2017-10-15

Rated 3 out of 5 by LeeSmith from Trial Report sc-398715 I bought this antibody(sc-398715) a month ago and it's pity that there was no flaser. Maybe the protein I dectected was not form the model organism-mouse so there are many different amino acid sequences in this protein.

Date published: 2017-09-25

caspase-1 p20 Antibody (D-4) Reviews - page 2

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