CARP Antibody (E-3): sc-398139

CARP Antibody (E-3) is a mouse monoclonal IgG1 antibody that detects CARP (cardiac ankyrin repeat protein) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CARP is a crucial nuclear protein involved in the regulation of cardiac gene expression, particularly in cardiac hypertrophy, where CARP expression is significantly elevated in conditions such as abdominal aorta constriction and end-stage heart failure. CARP plays a vital role in inhibiting the transcription of key cardiac markers, including cardiac troponin C and atrial natriuretic factor, by acting downstream of the cardiac homeobox gene Nkx2.5. Notably, CARP′s interaction with the major calcium-binding protein of cardiac sarcoplasmic reticulum, Calsequestrin, suggests a complex regulatory mechanism that may contribute to cardiac hypertrophy and fibrosis. Furthermore, CARP is induced by transforming growth factor beta (TGFβ) in vascular smooth muscle cells, where CARP may mediate TGFβ′s inhibitory effects on cell proliferation, highlighting CARP′s importance in both cardiac and vascular health. With specificity and versatility, anti-CARP antibody (E-3) is an essential tool for researchers investigating cardiac function and pathology.

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CARP Antibody (E-3) References:

1. Cardiac ankyrin repeat protein, a negative regulator of cardiac gene expression, is augmented in human heart failure.  |  Zolk, O., et al. 2002. Biochem Biophys Res Commun. 293: 1377-82. PMID: 12054667
2. Cardiac ankyrin repeat protein (CARP) expression in human and murine atherosclerotic lesions: activin induces CARP in smooth muscle cells.  |  de Waard, V., et al. 2003. Arterioscler Thromb Vasc Biol. 23: 64-8. PMID: 12524226
3. Cardiac ankyrin repeat protein is preferentially induced in atrophic myofibers of congenital myopathy and spinal muscular atrophy.  |  Nakada, C., et al. 2003. Pathol Int. 53: 653-8. PMID: 14516314
4. Dimerization of the cardiac ankyrin protein CARP: implications for MARP titin-based signaling.  |  Witt, SH., et al. 2005. J Muscle Res Cell Motil. 26: 401-8. PMID: 16450059
5. Expression of cardiac ankyrin repeat protein, CARP, in malignant tumors: diagnostic use of CARP protein immunostaining in rhabdomyosarcoma.  |  Ishiguro, N., et al. 2008. Hum Pathol. 39: 1673-9. PMID: 18656235
6. Cardiac ankyrin repeat protein is a marker of skeletal muscle pathological remodelling.  |  Laure, L., et al. 2009. FEBS J. 276: 669-84. PMID: 19143834
7. Upregulated expression of cardiac ankyrin repeat protein in human failing hearts due to arrhythmogenic right ventricular cardiomyopathy.  |  Wei, YJ., et al. 2009. Eur J Heart Fail. 11: 559-66. PMID: 19359327
8. Cardiac ankyrin repeat protein gene (ANKRD1) mutations in hypertrophic cardiomyopathy.  |  Arimura, T., et al. 2009. J Am Coll Cardiol. 54: 334-42. PMID: 19608031
9. A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein.  |  Kojic, S., et al. 2010. Arch Biochem Biophys. 502: 60-7. PMID: 20599664
10. Cardiac ankyrin repeat protein attenuates cardiac hypertrophy by inhibition of ERK1/2 and TGF-β signaling pathways.  |  Song, Y., et al. 2012. PLoS One. 7: e50436. PMID: 23227174

The preferred CARP antibody is CARP Antibody (G-2): sc-365056.