CARP Antibody (G-2): sc-365056

CARP Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CARP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CARP (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cardiac ankyrin repeat protein (CARP) plays a crucial role in regulating cardiac gene expression, particularly in response to stress conditions such as cardiac hypertrophy. Located primarily in the nucleus, CARP is essential for modulating transcriptional activity of genes involved in cardiac function. CARP expression is significantly upregulated in pathological conditions, such as in rats with abdominal aorta constriction and in patients with end-stage heart failure, highlighting CARP′s potential as a genetic marker for cardiac hypertrophy. Notably, CARP interacts with cardiac homeobox gene Nkx2.5, inhibiting its transactivation of the atrial natriuretic factor promoter, which is vital for maintaining normal cardiac function. Additionally, CARP expression is influenced by Calsequestrin, a major calcium-binding protein in cardiac sarcoplasmic reticulum, which may further link CARP to the development of cardiac hypertrophy and fibrosis. Furthermore, transforming growth factor beta (TGFβ) has been shown to induce CARP expression in vascular smooth muscle cells, suggesting CARP may play a role in mediating TGFβ′s inhibitory effects on cell proliferation, thereby contributing to cardiovascular health.

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CARP Antibody (G-2) References:

1. Cardiac ankyrin repeat protein is a novel marker of cardiac hypertrophy: role of M-CAT element within the promoter.  |  Aihara, Y., et al. 2000. Hypertension. 36: 48-53. PMID: 10904011
2. Transforming growth factor-beta/Smads signaling induces transcription of the cell type-restricted ankyrin repeat protein CARP gene through CAGA motif in vascular smooth muscle cells.  |  Kanai, H., et al. 2001. Circ Res. 88: 30-6. PMID: 11139470
3. Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies.  |  Bang, ML., et al. 2001. J Cell Biol. 153: 413-27. PMID: 11309420
4. Cardiac ankyrin repeat protein, a negative regulator of cardiac gene expression, is augmented in human heart failure.  |  Zolk, O., et al. 2002. Biochem Biophys Res Commun. 293: 1377-82. PMID: 12054667
5. Modulation of gene expression in transgenic mouse hearts overexpressing calsequestrin.  |  Ihara, Y., et al. 2002. Cell Calcium. 32: 21-9. PMID: 12127059
6. CARP, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue.  |  Shi, Y., et al. 2005. Am J Pathol. 166: 303-12. PMID: 15632022
7. Multifunctional protein: cardiac ankyrin repeat protein.  |  Zhang, N., et al. 2016. J Zhejiang Univ Sci B. 17: 333-41. PMID: 27143260
8. Cardiac ankyrin repeat protein attenuates cardiomyocyte apoptosis by upregulation of Bcl-2 expression.  |  Zhang, N., et al. 2016. Biochim Biophys Acta. 1863: 3040-3049. PMID: 27713078
9. Altered regulation of cardiac ankyrin repeat protein in heart failure.  |  Kempton, A., et al. 2018. Heliyon. 4: e00514. PMID: 29560432
10. Cardiac ankyrin repeat protein contributes to dilated cardiomyopathy and heart failure.  |  Zhang, N., et al. 2021. FASEB J. 35: e21488. PMID: 33734499
11. CARP, a cardiac ankyrin repeat protein, is downstream in the Nkx2-5 homeobox gene pathway.  |  Zou, Y., et al. 1997. Development. 124: 793-804. PMID: 9043061
12. A novel cardiac-restricted target for doxorubicin. CARP, a nuclear modulator of gene expression in cardiac progenitor cells and cardiomyocytes.  |  Jeyaseelan, R., et al. 1997. J Biol Chem. 272: 22800-8. PMID: 9278441

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CARP Antibody (G-2) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-CARP Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CARP Antibody (G-2) Specifications

Alternate Product Name: CARP

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-120

Epitope Target: CARP

Epitope Species: human

Product Reactivity/Detection: CARP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for CARP Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin