Anti-BRD3 Antibody (H-5) recommended for detection of BRD3 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-BRD3 Antibody (H-5): sc-515729

Anti-BRD3 Antibody (H-5) is rated 5.0 out of 5 by 1.
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Datasheets

    • BRD3 Antibody (H-5) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 176-192 within an internal region of BRD3 of human origin
    • recommended for detection of BRD3 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for BRD3 Antibody (H-5) for WB applications. These reagents are now offered in bundles with BRD3 Antibody (H-5) (see ordering information below).
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I need to use an antibody to detect the protein in samples of porcine origin. Do you know if BRD3 (H-5): sc-515729 mouse monoclonal antibody will work?

Asked by: TinTin
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from the effect is very good Overlay histogram showing HeLa cells stained with ab56342 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22ºC.
Date published: 2017-02-10
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