β-casein Antibody (F20.14): sc-53189

β-casein Antibody (F20.14) is a mouse monoclonal IgG1 kappa light chain antibody that detects beta-casein of human origin by western blotting (WB) and immunofluorescence (IF). β-casein Antibody (F20.14) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Beta-casein plays a vital role in newborn mammal nutrition as one of the primary proteins found in mammalian milk, particularly human milk, where beta-casein exists alongside κ-casein. Beta-casein′s unique structure, characterized by high phosphorylation and calcium-binding capacity, is crucial for forming micelles that enhance milk protein digestibility in neonates. The β-casein/κ-casein ratio is particularly significant in colostrum, which has a higher ratio than transitional and mature milk, facilitating better nutrient absorption during the critical early days of life. The human β-casein gene (Bca) is regulated by a network of transcription factors, including Stat5 and C/EBPb, which respond to lactogenic hormones, ensuring beta-casein production meets infant nutritional needs. Additionally, β-casein gene expression is influenced by NFkB, which acts as a negative regulator during pregnancy, highlighting the complex interplay of factors governing beta-casein synthesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

β-casein Antibody (F20.14) References:

1. Changes in the kappa-casein and beta-casein concentrations in human milk during lactation.  |  Cuillière, ML., et al. 1999. J Clin Lab Anal. 13: 213-8. PMID: 10494129
2. Autocrine insulin-like growth factor II inhibits beta-casein mRNA expression in a mammary cell line.  |  Lykos, MA., et al. 2000. J Dairy Sci. 83: 285-95. PMID: 10714862
3. Cooperative effects of STAT5 (signal transducer and activator of transcription 5) and C/EBPbeta (CCAAT/enhancer-binding protein-beta) on beta-casein gene transcription are mediated by the glucocorticoid receptor.  |  Wyszomierski, SL. and Rosen, JM. 2001. Mol Endocrinol. 15: 228-40. PMID: 11158330
4. Beta-casein variants and atherosclerosis-claims are premature.  |  Mann, J. and Skeaff, M. 2003. Atherosclerosis. 170: 11-2. PMID: 12957677
5. Regional localization of human beta-casein gene (CSN2) to 4pter-q21.  |  Menon, RS., et al. 1992. Genomics. 13: 225-6. PMID: 1577486
6. Cloning and sequencing of a cDNA encoding human milk beta-casein.  |  Lönnerdal, B., et al. 1990. FEBS Lett. 269: 153-6. PMID: 2387396
7. Effects of milk containing only A2 beta casein versus milk containing both A1 and A2 beta casein proteins on gastrointestinal physiology, symptoms of discomfort, and cognitive behavior of people with self-reported intolerance to traditional cows' milk.  |  Jianqin, S., et al. 2016. Nutr J. 15: 35. PMID: 27039383
8. Nanoparticle Tracking Analysis of β-Casein Nanocarriers.  |  Masri, H. and Raynes, JK. 2020. Methods Mol Biol. 2073: 301-309. PMID: 31612448
9. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin.  |  Burchell, J., et al. 1985. Hybridoma. 4: 341-50. PMID: 3905581
10. Human beta-casein. Amino acid sequence and identification of phosphorylation sites.  |  Greenberg, R., et al. 1984. J Biol Chem. 259: 5132-8. PMID: 6715339
11. Structure of the human beta-casein encoding gene.  |  Hansson, L., et al. 1994. Gene. 139: 193-9. PMID: 8112603
12. A distal enhancer region in the human beta-casein gene mediates the response to prolactin and glucocorticoid hormones.  |  Winklehner-Jennewein, P., et al. 1998. Gene. 217: 127-39. PMID: 9795185

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-β-casein Antibody (F20.14) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-β-casein Antibody (F20.14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

β-casein Antibody (F20.14) Specifications

Alternate Product Name: CSN2

Product Clone Name: F20.14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: casein

Epitope Species: human

Product Reactivity/Detection: β casein (29kDa)

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for β-casein Antibody (F20.14): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin