ASS1 Antibody (E-12): sc-365475

ASS1 Antibody (E-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects ASS1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ASS1 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASS1, also known as argininosuccinate synthase or citrulline-aspartate ligase, plays a crucial role in the urea cycle, catalyzing the second-to-last step in the arginine biosynthetic pathway. This enzyme is essential for the detoxification of ammonia in the liver, converting citrulline and aspartate into argininosuccinate, which is subsequently cleaved to produce arginine and fumarate. A deficiency in ASS1 can lead to citrullinemia (CTLN1), an autosomal recessive disorder characterized by severe vomiting spells and cognitive impairments, highlighting the importance of ASS1 in metabolic health. The tetrameric structure of ASS1, composed of identical subunits, is vital for enzymatic function, as this composition allows for proper substrate binding and catalytic activity, making ASS1 protein interactions critical for understanding related metabolic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ASS1 Antibody (E-12) References:

1. Structure of the human argininosuccinate synthetase gene and an improved system for molecular diagnostics in patients with classical and mild citrullinemia.  |  Häberle, J., et al. 2002. Hum Genet. 110: 327-33. PMID: 11941481
2. Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo.  |  Hao, G., et al. 2004. J Biol Chem. 279: 36192-200. PMID: 15192091
3. Identification of salt-sensitive genes in the kidneys of Dahl rats.  |  Lighthall, GK., et al. 2004. J Hypertens. 22: 1487-94. PMID: 15257170
4. Pregnancy in a healthy woman with untreated citrullinemia.  |  Potter, MA., et al. 2004. Am J Med Genet A. 129A: 77-82. PMID: 15266621
5. Assignment of CPS1, OTC, CRYD2, ARG2 and ASS genes to the chicken RH map.  |  Shimogiri, T., et al. 2004. Genet Sel Evol. 36: 593-9. PMID: 15339635
6. Citrulline can preserve proliferation and prevent the loss of CD3 zeta chain under conditions of low arginine.  |  Bansal, V., et al. 2004. JPEN J Parenter Enteral Nutr. 28: 423-30. PMID: 15568289
7. A pregnant patient with fulminant hepatic failure was found to carry a novel missense mutation in the argininosuccinate synthetase gene.  |  Ito, S., et al. 2004. J Gastroenterol. 39: 1115-7. PMID: 15580409
8. Accessibility of endothelial and inducible nitric oxide synthase to the intracellular citrulline-arginine regeneration pathway.  |  Shen, LJ., et al. 2005. Biochem Pharmacol. 69: 97-104. PMID: 15588718
9. Identification of essential arginine residue(s) for Mg-ATP binding of human argininosuccinate synthetase.  |  Isashiki, Y., et al. 1989. Protein Seq Data Anal. 2: 283-7. PMID: 2788888
10. Sequence for human argininosuccinate synthetase cDNA.  |  Bock, HG., et al. 1983. Nucleic Acids Res. 11: 6505-12. PMID: 6194510
11. Molecular structures of human argininosuccinate synthetase pseudogenes. Evolutionary and mechanistic implications.  |  Freytag, SO., et al. 1984. J Biol Chem. 259: 3160-6. PMID: 6321498

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ASS1 Antibody (E-12) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-ASS1 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ASS1 Antibody (E-12) Specifications

Alternate Product Name: ASS1

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-231

Epitope Target: ASS1

Epitope Species: human

Product Reactivity/Detection: ASS1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for ASS1 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin