ARL5B Antibody (E-3): sc-393511

ARL5B Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARL5B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ARL5B antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARL5B, also known as ADP-ribosylation factor-like protein 5B or ARL8, is a 179 amino acid protein that plays a crucial role in cellular processes by participating in vesicular trafficking pathways, which are essential for the transport of proteins and lipids within cells. ARL5B is predominantly expressed in the brain, heart, lung, cartilage, and kidney, but is absent in the spleen, highlighting specific functional roles in various tissues. ARL5B shares 80% sequence identity with ARL5 and exists in two isoforms due to alternative splicing, which may contribute to functional diversity. ARL5B involvement in the activation of phospholipase D, a key enzyme in lipid signaling pathways, influences cellular responses to external stimuli.

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ARL5B Antibody (E-3) References:

1. Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein.  |  Secombe, J. and Parkhurst, SM. 2004. J Biol Chem. 279: 17126-33. PMID: 14871887
2. Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation.  |  Okai, T., et al. 2004. J Cell Sci. 117: 4705-15. PMID: 15331635
3. Arf-like GTPases: not so Arf-like after all.  |  Burd, CG., et al. 2004. Trends Cell Biol. 14: 687-94. PMID: 15564045
4. Arf family GTPases: roles in membrane traffic and microtubule dynamics.  |  Kahn, RA., et al. 2005. Biochem Soc Trans. 33: 1269-72. PMID: 16246095
5. Nomenclature for the human Arf family of GTP-binding proteins: ARF, ARL, and SAR proteins.  |  Kahn, RA., et al. 2006. J Cell Biol. 172: 645-50. PMID: 16505163
6. An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility.  |  Hofmann, I. and Munro, S. 2006. J Cell Sci. 119: 1494-503. PMID: 16537643
7. The Arf-family protein, Arl8b, is involved in the spatial distribution of lysosomes.  |  Bagshaw, RD., et al. 2006. Biochem Biophys Res Commun. 344: 1186-91. PMID: 16650381
8. Expression of ADP-ribosylation factor-like protein 8B mRNA in the brain is down-regulated in mice fed a high-fat diet.  |  Haraguchi, T., et al. 2006. Biosci Biotechnol Biochem. 70: 1798-802. PMID: 16861820
9. Arl5b is a Golgi-localised small G protein involved in the regulation of retrograde transport.  |  Houghton, FJ., et al. 2012. Exp Cell Res. 318: 464-77. PMID: 22245584
10. The FTO/miR-181b-3p/ARL5B signaling pathway regulates cell migration and invasion in breast cancer.  |  Xu, Y., et al. 2020. Cancer Commun (Lond). 40: 484-500. PMID: 32805088
11. Knockdown of ARL5B Induces Mitochondrial-mediated Apoptosis and Inhibits Glycolysis in Breast Cancer Cells by Activating MDA5 Signaling.  |  Zhang, L., et al. 2022. Curr Cancer Drug Targets. 22: 843-853. PMID: 35546774
12. ARL5b inhibits human rhinovirus 16 propagation and impairs macrophage-mediated bacterial clearance.  |  Faure-Dupuy, S., et al. 2024. EMBO Rep. 25: 1156-1175. PMID: 38332148

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARL5B Antibody (E-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ARL5B Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARL5B Antibody (E-3) Specifications

Alternate Product Name: ARL5B

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-179

Epitope Target: ARL5B

Epitope Species: human

Product Reactivity/Detection: ARL5B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ARL5B Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ARL5A/5B/5C antibody is ARL5A/5B/5C Antibody (D-7): sc-390269.