ARK-2 Antibody (13E8A7): sc-65987

ARK-2 Antibody (13E8A7) is a mouse monoclonal IgG1 antibody that detects ARK-2 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. ARK-2, or aurora related kinase 2, is a centrosome-associated serine/threonine kinase that plays a crucial role in regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis, making ARK-2 essential for proper cell division and genomic stability. ARK-2 is predominantly localized in the nucleus, where ARK-2 associates with distinct components of the mitotic machinery, such as the spindle poles and midbody, highlighting ARK-2′s importance in the orchestration of mitotic events. Notably, ARK-2 protein levels peak during the S and G2/M phases of the cell cycle, indicating ARK-2′s active involvement in cell cycle progression, while ARK-2′s unique genetic locus suggests distinct functions compared to ARK-1. Furthermore, ARK-2 undergoes post-translational modification through phosphorylation at Threonine 232, a process critical for activation and function, underscoring the significance of such modifications in regulating kinase activity and cellular processes. With specific reactivity and versatile applications, anti-ARK-2 antibody (13E8A7) is an invaluable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying mitotic regulation.

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ARK-2 Antibody (13E8A7) References:

1. EBNA3C regulates p53 through induction of Aurora kinase B.  |  Jha, HC., et al. 2015. Oncotarget. 6: 5788-803. PMID: 25691063
2. Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells.  |  Li, M., et al. 2017. J Mol Biol. 429: 2042-2045. PMID: 28137420
3. Aurora kinase B-phosphorylated HP1α functions in chromosomal instability.  |  Williams, MM., et al. 2019. Cell Cycle. 18: 1407-1421. PMID: 31130069
4. Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.  |  Kasam, RK., et al. 2020. EMBO Mol Med. 12: e12131. PMID: 32761869
5. Aurora Kinase B Inhibition: A Potential Therapeutic Strategy for Cancer.  |  Borah, NA. and Reddy, MM. 2021. Molecules. 26: PMID: 33915740
6. Mitotic syndicates Aurora Kinase B (AURKB) and mitotic arrest deficient 2 like 2 (MAD2L2) in cohorts of DNA damage response (DDR) and tumorigenesis.  |  Marima, R., et al. 2021. Mutat Res Rev Mutat Res. 787: 108376. PMID: 34083040
7. Sustained Aurora Kinase B Expression Confers Resistance to PI3K Inhibition in Head and Neck Squamous Cell Carcinoma.  |  Shah, PA., et al. 2022. Cancer Res. 82: 4444-4456. PMID: 36169922
8. Effect of Aurora kinase B on polyploidy and decidualization in mouse uterus.  |  Wang, PC., et al. 2023. Am J Reprod Immunol. 90: e13793. PMID: 37881124
9. Determinants of Aurora kinase B inhibitor sensitivity in small cell lung cancer.  |  Duan, L. and Maki, CG. 2024. Transl Lung Cancer Res. 13: 223-228. PMID: 38496702
10. Aurora kinase B inhibition in small-cell lung cancer: BCL-2 as a potential therapeutic biomarker and combination target.  |  Hosoya, K. and Ozasa, H. 2024. Transl Lung Cancer Res. 13: 689-693. PMID: 38601438

The preferred ARK-2 antibody is ARK-2 Antibody (A-3): sc-393357.