ARK-2 Antibody (A-3): sc-393357

ARK-2 Antibody (A-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects ARK-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARK-2 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARK-2, also known as Aurora B Kinase, plays a critical role in the regulation of mitosis, particularly in chromosome alignment and segregation, which is essential for maintaining genomic stability. Located at the midbody during cell division, ARK-2 ensures proper cytokinesis and prevents aneuploidy, a condition that can lead to cancer. ARK-2 expression peaks during the S and G2/M phases of the cell cycle, highlighting ARK-2′s importance in the later stages of cell division. Additionally, ARK-2 interacts with several key proteins involved in the mitotic process, including INCENP and survivin, which are crucial for the formation of the chromosomal passenger complex. This complex is vital for the correct positioning of the mitotic spindle and the successful completion of cell division. The unique expression patterns and interactions of ARK-2 underscore ARK-2′s potential as a therapeutic target in cancer treatment, making anti-ARK-2 antibody (A-3) an invaluable tool for researchers studying cell cycle dynamics and cancer biology.

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ARK-2 Antibody (A-3) References:

1. Cdc20 associates with the kinase aurora2/Aik.  |  Farruggio, DC., et al. 1999. Proc Natl Acad Sci U S A. 96: 7306-11. PMID: 10377410
2. Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases.  |  Giet, R. and Prigent, C. 1999. J Cell Sci. 112 (Pt 21): 3591-601. PMID: 10523496
3. Degradation of human Aurora2 protein kinase by the anaphase-promoting complex-ubiquitin-proteasome pathway.  |  Honda, K., et al. 2000. Oncogene. 19: 2812-9. PMID: 10851084
4. An Aurora Kinase B-Based Mouse System to Efficiently Identify and Analyze Proliferating Cardiomyocytes.  |  Fu, W., et al. 2020. Front Cell Dev Biol. 8: 570252. PMID: 33117800
5. Aurora Kinase B Expression, Its Regulation and Therapeutic Targeting in Human Retinoblastoma.  |  Borah, NA., et al. 2021. Invest Ophthalmol Vis Sci. 62: 16. PMID: 33704359
6. Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.  |  Aboelenain, M. and Schindler, K. 2021. Development. 148: PMID: 34636397
7. Role of aurora kinase B in regulating resistance to paclitaxel in breast cancer cells.  |  Liu, M., et al. 2022. Hum Cell. 35: 678-693. PMID: 35088239
8. Targeting BCL2 Overcomes Resistance and Augments Response to Aurora Kinase B Inhibition by AZD2811 in Small Cell Lung Cancer.  |  Ramkumar, K., et al. 2023. Clin Cancer Res. 29: 3237-3249. PMID: 37289191
9. cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2.  |  Shindo, M., et al. 1998. Biochem Biophys Res Commun. 244: 285-92. PMID: 9514916
10. A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.  |  Bischoff, JR., et al. 1998. EMBO J. 17: 3052-65. PMID: 9606188
11. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.  |  Zhou, H., et al. 1998. Nat Genet. 20: 189-93. PMID: 9771714
12. Identification and characterization of STK12/Aik2: a human gene related to aurora of Drosophila and yeast IPL1.  |  Kimura, M., et al. 1998. Cytogenet Cell Genet. 82: 147-52. PMID: 9858806

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARK-2 Antibody (A-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ARK-2 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARK-2 Antibody (A-3) Specifications

Alternate Product Name: ARK-2

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 48-79

Epitope Target: ARK-2

Epitope Species: human

Product Reactivity/Detection: ARK-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ARK-2 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin