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Aquaporin 1/AQP1 Antibody (1/22): sc-32737

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Datasheets
  • Aquaporin 1/AQP1 Antibody (1/22) is a mouse monoclonal IgG2b κ, cited in 34 publications, provided at 200 µg/ml
  • raised against amino acids 249-269 of AQP1 of rat origin
  • Anti-Aquaporin 1/AQP1 Antibody (1/22) is recommended for detection of AQP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-Aquaporin 1/AQP1 Antibody (1/22) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Aquaporin 1/AQP1 Antibody (1/22). This work is in progress.

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AQP1 Antibody (1/22) is a mouse monoclonal IgG2b kappa light chain antibody that detects AQP1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). Anti-AQP1 antibody (1/22) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. AQP1, a member of the aquaporin family, plays a crucial role in facilitating water transport across cell membranes, which is essential for maintaining cellular homeostasis and regulating fluid balance in tissues. This integral membrane protein is predominantly located in erythrocytes and renal tubule cells, where AQP1 is vital for renal water absorption and the generation of bodily fluids. AQP1′s presence in these locations underscores AQP1′s importance in physiological processes such as urine concentration and blood volume maintenance. Additionally, AQP1′s ability to transport not only water but also small solutes like glycerol and urea in certain isoforms highlights AQP1′s versatile role in cellular transport mechanisms. The diverse expression of aquaporins across different tissues and their functional implications make anti-AQP1 antibody (1/22) an invaluable tool for researchers studying fluid transport and related physiological processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Aquaporin 1/AQP1 Antibody (1/22)

sc-32737
200 µg/ml
$316.00

Aquaporin 1/AQP1 Antibody (1/22) AC

sc-32737 AC
500 µg/ml, 25% agarose
$416.00

Aquaporin 1/AQP1 Antibody (1/22) HRP

sc-32737 HRP
200 µg/ml
$316.00

Aquaporin 1/AQP1 Antibody (1/22) FITC

sc-32737 FITC
200 µg/ml
$330.00

Aquaporin 1/AQP1 Antibody (1/22) PE

sc-32737 PE
200 µg/ml
$343.00

Aquaporin 1/AQP1 Antibody (1/22) Alexa Fluor® 488

sc-32737 AF488
200 µg/ml
$357.00

Aquaporin 1/AQP1 Antibody (1/22) Alexa Fluor® 546

sc-32737 AF546
200 µg/ml
$357.00

Aquaporin 1/AQP1 Antibody (1/22) Alexa Fluor® 594

sc-32737 AF594
200 µg/ml
$357.00

Aquaporin 1/AQP1 Antibody (1/22) Alexa Fluor® 647

sc-32737 AF647
200 µg/ml
$357.00

Aquaporin 1/AQP1 Antibody (1/22) Alexa Fluor® 680

sc-32737 AF680
200 µg/ml
$357.00

Aquaporin 1/AQP1 Antibody (1/22) Alexa Fluor® 790

sc-32737 AF790
200 µg/ml
$357.00

i antigen retrieval required?

Asked by: wwwy
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this antibody.
Answered by: Technical Support
Date published: 2019-06-16

Hi, does Anti Aquaporin-1 (1/22) come with BSA or it is possible to have a BSA-free sample? Best, Maria

Asked by: Maria Elena
Thank you for your question. There is no BSA in the antibody buffer. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-01-26

Which secondary should be used to detect this mouse monoclonal primary antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 14
Date published: 2017-01-20
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Rated 4 out of 5 by from work well in WB at 1:200, with hippocampuswork well in WB at 1:200, with hippocampus as photo shows
Date published: 2018-05-10
Rated 5 out of 5 by from better resultThis monoclonaly antibody shows more better clear band than polyclonal antibody,it's good quality for every aspect
Date published: 2017-06-15
Rated 5 out of 5 by from published resultsThis antibody provides strong and clear detection in at kidney tissue extracts
Date published: 2017-05-18
Rated 5 out of 5 by from Superb productGreat for multiple applications including WB, IF and IHC
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of AQP1 expression in mouse kidney and rat kidney tissue extracts.
Date published: 2017-01-12
Rated 5 out of 5 by from Worked well in IF in KNRK cells with strongWorked well in IF in KNRK cells with strong membrane localization.
Date published: 2015-04-09
Rated 5 out of 5 by from Strong band in WB in mouse and rat kidneyStrong band in WB in mouse and rat kidney tissue extracts. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Strong membrane staining of centroacinarStrong membrane staining of centroacinar cells in human pancreas tissue in IHC. -SCBT QC
Date published: 2015-01-06
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Aquaporin 1/AQP1 Antibody (1/22) is rated 4.9 out of 5 by 10.
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