AQP1 Antibody (B-11) recommended for detection of AQP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

AQP1 Antibody (B-11): sc-25287

AQP1 Antibody (B-11) is rated 4.7 out of 5 by 19.
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Datasheets

    • Anti-AQP1 Antibody (B-11) is a mouse monoclonal IgG1 κ AQP1 antibody, cited in 65 publications, provided at 200 µg/ml
    • raised against amino acids 215-269 of AQP1 of human origin
    • Anti-AQP1 Antibody (B-11) is recommended for detection of AQP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-AQP1 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AQP1 (B-11): sc-25287.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for AQP1 Antibody (B-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with AQP1 Antibody (B-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can I use AQP1 (B-11) for immunofluorescence staining of mice renal tissue? If so, is there a recommended dilution?

Asked by: Loz2
Thank you for your question. This mouse monoclonal antibody, AQP1 (B-11): sc-25287, is recommended for immunofluorescence staining (IF) of mouse tissues. We recommend a starting dilution of 1:50 (range of 1:50-1:500). A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-10-02

Can I use AQP1 (B-11): sc-25287 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated monoclonal antibody. AQP1 (B-11): sc-25287 monoclonal antibody is available directly conjugated to either HRP (sc-25287 HRP), phycoerythrin (sc-25287 PE), fluorescein (sc-25287 FITC), Alexa Fluor® 488 (sc-25287 AF488) or Alexa Fluor® 647 (sc-25287 AF647)
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 4 out of 5 by from Works fine Clear band visible ~40 kDa when used at 1:200 in 2% Milk (ON 4 degrees, gradient gel 4-15%).
Date published: 2020-01-02
Rated 5 out of 5 by from very good AQP1 (B-11): sc-25287. tested using in adipose tissue homogenate enriched in capillaries. Antibody dilution: 1 : 250.
Date published: 2018-10-30
Rated 5 out of 5 by from work well in WB at 1:200, with hippocampus work well in WB at 1:200, with hippocampus,i like this
Date published: 2018-05-10
Rated 5 out of 5 by from Good Antibody - Specific I got a clean band at the right size for HK2 cells
Date published: 2018-02-05
Rated 4 out of 5 by from Working fine in WB This AQP1 antibody is working fine in WB with human liver, mouse liver, kidney, brain and hippocampus. However, it does not recognize human origin of AQP1.
Date published: 2017-10-12
Rated 4 out of 5 by from Aqp1 Great western results in ES mouse cells; strong, specific bands.
Date published: 2017-10-03
Rated 4 out of 5 by from Good product I bought this antibody for human primary TM cells staining. I used 1 in 100 duilution and it was worked well in my case.
Date published: 2017-09-11
Rated 5 out of 5 by from Good immunofluorescence results tested the antibody in HK-2 cells, I got excellent immunofluorescence results
Date published: 2017-07-08
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