Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6): sc-74519

AChRα4 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects AChR alpha 4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AChRα4 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AChR alpha 4 protein, also known as CHRNA4, is a critical component of the nicotinic acetylcholine receptor, playing a vital role in neurotransmission at the neuromuscular junction and in the central nervous system. CHRNA4 is a multi-pass membrane protein consisting of 627 amino acids and is essential for proper synaptic transmission, forming a transmembrane aqueous pore that allows ions to flow in response to acetylcholine binding. CHRNA4′s structural integrity is crucial, as alterations can lead to dysfunction in synaptic signaling, contributing to conditions such as nocturnal frontal lobe epilepsy type 1 (ENFL1). This autosomal dominant epilepsy is characterized by nocturnal seizures and hyperkinetic automatisms, underscoring CHRNA4′s importance in maintaining normal neurological function. Anti-AChRα4 antibody (A-6) serves as a valuable tool for researchers investigating the mechanisms of synaptic transmission and related disorders.

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Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6) References:

1. A novel SSCP variant (c.828G>A) within the M2 domain of the human neuronal nicotinic acetylcholine receptor alpha 4 subunit gene, CHRNA4.  |  Akiyoshi, H., et al. 2000. Hum Mutat. 16: 450. PMID: 11058920
2. Mutation (Ser284Leu) of neuronal nicotinic acetylcholine receptor alpha 4 subunit associated with frontal lobe epilepsy causes faster desensitization of the rat receptor expressed in oocytes.  |  Matsushima, N., et al. 2002. Epilepsy Res. 48: 181-6. PMID: 11904236
3. A common haplotype of the nicotine acetylcholine receptor alpha 4 subunit gene is associated with vulnerability to nicotine addiction in men.  |  Feng, Y., et al. 2004. Am J Hum Genet. 75: 112-21. PMID: 15154117
4. Probing conformational changes in the nicotinic acetylcholine receptor by Fourier transform infrared difference spectroscopy.  |  Baenziger, JE., et al. 1992. Biophys J. 62: 64-6. PMID: 1600101
5. Ligand-gated ion channels in the brain: the amino acid receptor superfamily.  |  Betz, H. 1990. Neuron. 5: 383-92. PMID: 1698394
6. Acetylcholinesterase reactivators modify the functional properties of the nicotinic acetylcholine receptor ion channel.  |  Alkondon, M., et al. 1988. J Pharmacol Exp Ther. 245: 543-56. PMID: 2452874
7. Differentiation of Nsc-34 cells is characterized by expression of NGF receptor p75, glutaminase and NCAM L1, activation of mitochondria, and sensitivity to fatty acid intervention.  |  Keilhoff, G., et al. 2020. Acta Histochem. 122: 151574. PMID: 32622426
8. Neurotransmitter action: opening of ligand-gated ion channels.  |  Unwin, N. 1993. Cell. 72 Suppl: 31-41. PMID: 7679054
9. The diversity of neuronal nicotinic acetylcholine receptors.  |  Sargent, PB. 1993. Annu Rev Neurosci. 16: 403-43. PMID: 7681637
10. Quantal release of neurotransmitter and long-term potentiation.  |  Stevens, CF. 1993. Cell. 72 Suppl: 55-63. PMID: 8094037
11. The role of NMDA receptors in information processing.  |  Daw, NW., et al. 1993. Annu Rev Neurosci. 16: 207-22. PMID: 8460891
12. Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels.  |  Ramirez-Latorre, J., et al. 1996. Nature. 380: 347-51. PMID: 8598930

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6) Specifications

Alternate Product Name: CHRNA4

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 342-474

Epitope Target: AChRα4

Epitope Species: human

Product Reactivity/Detection: AChRα4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin