Chemical activators of ZNF682 can regulate its activity through various biochemical pathways. Phorbol 12-myristate 13-acetate (PMA) is one such activator that exerts its effect by activating protein kinase C (PKC), which in turn phosphorylates ZNF682, leading to its functional activation. Similarly, Forskolin raises intracellular cAMP levels, which activates protein kinase A (PKA). PKA then phosphorylates ZNF682, enhancing its activity. Ionomycin acts by increasing intracellular calcium levels, which activates calcium-dependent kinases that also target and phosphorylate ZNF682, resulting in its activation. Conversely, Okadaic Acid and Calyculin A function by inhibiting protein phosphatases such as PP1 and PP2A, which normally dephosphorylate proteins like ZNF682. This inhibition maintains ZNF682 in a phosphorylated, and thus active, state.
Furthermore, Anisomycin activates the mitogen-activated protein kinases (MAPKs) pathway, which includes kinases capable of phosphorylating ZNF682 to activate it. Thapsigargin disrupts calcium homeostasis, indirectly leading to the activation of kinases that can phosphorylate ZNF682. Epigallocatechin Gallate (EGCG) prevents the breakdown of cAMP by inhibiting phosphodiesterases, culminating in the activation of PKA, which can then activate ZNF682 through phosphorylation. Dibutyryl-cAMP, a stable cAMP analog, directly stimulates PKA, which phosphorylates and activates ZNF682. Phosphatidic Acid activates the mTOR signaling pathway, a route known to be involved in ZNF682 phosphorylation and activation. Staurosporine, at low concentrations, can activate kinases that phosphorylate ZNF682. Lastly, Bisindolylmaleimide I, while primarily known as a PKC inhibitor, can lead to the activation of compensatory pathways that phosphorylate and activate ZNF682, indicating a complex regulation of ZNF682 activity through multiple signaling cascades.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate ZNF682, leading to its activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium levels, activating calcium-dependent kinases that can phosphorylate and activate ZNF682. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid inhibits protein phosphatases PP1 and PP2A, maintaining ZNF682 in an activated phosphorylated state. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A, like Okadaic Acid, inhibits PP1 and PP2A, resulting in prolonged phosphorylation and activation of ZNF682. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin activates mitogen-activated protein kinases (MAPKs), which are involved in the phosphorylation and activation of ZNF682. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin disrupts calcium homeostasis, which can lead to the activation of kinases that phosphorylate and activate ZNF682. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP, a cAMP analog, directly stimulates PKA leading to the phosphorylation and activation of ZNF682. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $153.00 $396.00 | 113 | |
At low concentrations, Staurosporine can activate certain kinases that phosphorylate and activate ZNF682. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I selectively inhibits PKC, potentially leading to the activation of alternative pathways that phosphorylate and activate ZNF682. | ||||||