ZNF383 Activators
ZNF383 employ various cellular pathways to modulate its activity. Phorbol 12-myristate 13-acetate (PMA) and 1,2-Dioctanoyl-sn-glycerol (DiC8), both activators of protein kinase C (PKC), can enhance the phosphorylation and activation of ZNF383. PKC serves as a pivotal kinase that targets a wide array of proteins, including those that are involved in DNA binding and transcriptional regulation. Similarly, Tetradecanoylphorbol Acetate (TPA), another PKC activator, can also promote the phosphorylation and activation of ZNF383, underlining the role of PKC-mediated pathways in the functional modulation of this protein. Forskolin, by activating adenylate cyclase, raises the levels of cyclic AMP and subsequently activates protein kinase A (PKA), which can phosphorylate transcription factors such as ZNF383, thereby enhancing its DNA binding activity. Ionomycin, by acting as a calcium ionophore, raises intracellular calcium levels, which can activate calcium-dependent kinases capable of phosphorylating ZNF383, highlighting the importance of calcium signaling in the regulation of such proteins.
Epidermal Growth Factor (EGF) and Insulin both trigger tyrosine kinase signaling cascades through their respective receptors. Activation of these pathways can lead to the modification of various transcription factors by signaling molecules such as STAT or ERK for EGF, and the activation of the PI3K/Akt pathway for Insulin, which in turn can modulate the activity of ZNF383. Anisomycin, which activates the stress-activated protein kinases (SAPKs)/Jun amino-terminal kinases (JNKs), can lead to changes in ZNF383 activity, indicating the protein's involvement in stress response signaling. Compounds like Calyculin A and Okadaic Acid, inhibitors of protein phosphatases such as PP1 and PP2A, can indirectly maintain or enhance the phosphorylation state of ZNF383 by preventing its dephosphorylation. Thapsigargin, which inhibits the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), leads to increased cytosolic calcium levels and may activate kinases like CaMK that can phosphorylate ZNF383. Lastly, BAY 11-7082 inhibits NF-κB activation, which can alter the regulation of genes that interact with or regulate ZNF383, thus influencing its activation state within the cellular signaling network.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is known to phosphorylate a wide range of substrate proteins. Activation of PKC can lead to the phosphorylation and subsequent activation of ZNF383, as PKC-mediated phosphorylation is a common mechanism for activating zinc finger proteins involved in DNA binding and transcriptional regulation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that raises intracellular calcium levels, which can activate calcium-dependent protein kinases such as calmodulin-dependent kinase (CaMK). These kinases can then phosphorylate and activate ZNF383, as calcium signaling is crucial for the regulation of numerous zinc finger proteins. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin binds to its receptor, initiating a signal transduction cascade that activates phosphoinositide 3-kinase (PI3K) and downstream Akt kinase. Akt can phosphorylate a multitude of proteins, potentially including ZNF383, thus facilitating its activation and role in metabolic gene regulation. | ||||||
1,2-Dioctanoyl-sn-glycerol | 60514-48-9 | sc-202397 sc-202397A | 10 mg 50 mg | $47.00 $254.00 | 2 | |
DiC8 is a synthetic analog of diacylglycerol (DAG), an activator of PKC. By activating PKC, DiC8 could enhance the phosphorylation and activation of ZNF383, similar to the mechanism proposed for PMA. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a potent activator of the stress-activated protein kinases (SAPKs) / Jun amino-terminal kinases (JNKs). Activation of these kinases may lead to phosphorylation of ZNF383, enhancing its transcriptional activity in response to stress signals. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A), leading to increased phosphorylation levels of proteins. By inhibiting phosphatase activity, Calyculin A could indirectly increase the phosphorylation and activation state of ZNF383. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Similar to Calyculin A, Okadaic Acid inhibits PP1 and PP2A, which can result in the increased phosphorylation and activation of proteins including ZNF383, as it prevents the dephosphorylation and inactivation of the protein. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), leading to an increase in cytosolic calcium levels. Elevated calcium can activate kinases such as CaMK, which might then phosphorylate and activate ZNF383. | ||||||
BAY 11-7082 | 19542-67-7 | sc-200615B sc-200615 sc-200615A | 5 mg 10 mg 50 mg | $62.00 $85.00 $356.00 | 155 | |
BAY 11-7082 inhibits NF-κB activation by blocking the phosphorylation of IκBα. This can lead to altered regulation of genes controlled by NF-κB, some of which may interact with or regulate ZNF383, thus influencing its activation state through changes in cellular signaling dynamics. | ||||||