ZNF253 Activators
ZNF253 can initiate a cascade of intracellular events leading to its functional activation, which is crucial for its role in transcriptional regulation. Phorbol 12-myristate 13-acetate (PMA) and its analog 12-O-Tetradecanoylphorbol-13-acetate (TPA) are known to activate protein kinase C (PKC), a family of enzymes that phosphorylate various target proteins, including transcription factors. The activation of PKC through these chemicals can lead to the phosphorylation and subsequent activation of ZNF253, thereby enhancing its ability to regulate gene expression. Similarly, Forskolin, by elevating intracellular cyclic AMP (cAMP), triggers protein kinase A (PKA), which can also phosphorylate and activate ZNF253. This phosphorylation can alter ZNF253's conformation, modulating its interaction with DNA and other proteins within the transcriptional machinery.
Epidermal Growth Factor (EGF) sets off a signaling pathway involving MAPK/ERK, which could target ZNF253 for phosphorylation and activation. Insulin, through its receptor, activates the PI3K/Akt signaling pathway, which has various downstream effects, including the activation of proteins that can modify ZNF253's activity. The disruption of calcium homeostasis by chemicals such as Ionomycin and Thapsigargin can lead to the activation of calcium-dependent kinases, which, in turn, can phosphorylate ZNF253. Inhibition of protein phosphatases by Calyculin A and Okadaic Acid prevents the dephosphorylation of proteins, potentially leading to a sustained activation state of ZNF253. Anisomycin, a stress-activated protein kinase activator, can signal for the activation of ZNF253 through phosphorylation by stress-activated pathways. Lastly, Bryostatin 1, by modulating PKC, can activate ZNF253 through similar mechanisms as PMA and TPA, highlighting the central role of PKC-mediated phosphorylation in the regulation of ZNF253 activity. Each of these chemicals, by targeting specific kinases or phosphatases, ensures the activation of ZNF253, promoting its role in the regulation of gene expression.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate and thereby activate ZNF253 as part of a signal transduction pathway that governs transcriptional regulation. | ||||||
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin activates adenylyl cyclase, increasing cAMP levels, which in turn activate PKA. PKA can phosphorylate transcription factors and associated proteins, potentially including ZNF253, leading to its activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $76.00 $265.00 | 80 | |
Ionomycin acts as a calcium ionophore, raising intracellular calcium levels, which can activate calcium-dependent protein kinases that may target and activate ZNF253. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $153.00 $1224.00 $12239.00 | 82 | |
Insulin receptor activation initiates a cascade involving PI3K/Akt signaling that could lead to the activation of ZNF253 through post-translational modifications mediated by downstream kinases. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A sc-24000B sc-24000C | 10 µg 100 µg 500 µg 1 mg | $160.00 $750.00 $1400.00 $3000.00 | 59 | |
Calyculin A inhibits protein phosphatases PP1 and PP2A, which could result in the hyperphosphorylation and activation of proteins, potentially including ZNF253. | ||||||
L-α-Lecithin, Egg Yolk, Highly Purified | 8002-43-5 | sc-203096 | 250 mg | $78.00 | ||
Phosphatidylserine can modulate membrane-associated signaling pathways and may recruit and activate kinases that phosphorylate and activate ZNF253. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A, potentially leading to increased phosphorylation and activation of ZNF253 by kinases. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin activates stress-activated protein kinases (SAPKs), such as JNK, which may phosphorylate and activate ZNF253 as part of the cellular stress response. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $240.00 | 9 | |
Bryostatin 1 binds to and modulates PKC, potentially leading to phosphorylation and activation of ZNF253 within the PKC signaling pathway. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $94.00 $349.00 | 114 | |
Thapsigargin disrupts calcium homeostasis by inhibiting the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), potentially leading to the activation of calcium-dependent kinases that could phosphorylate and activate ZNF253. | ||||||