ZFP345 Activators
Chemical activators of ZFP345 can engage different signaling pathways to modulate the protein's activity via phosphorylation. Phorbol 12-myristate 13-acetate, for instance, activates protein kinase C, which is known for its broad substrate specificity and role in a variety of biological processes. Upon activation, PKC can phosphorylate ZFP345, thereby modulating its function. Forskolin operates through a distinct mechanism, raising intracellular cAMP levels, which in turn activates protein kinase A. PKA then targets ZFP345 among its substrates, phosphorylating and altering its activity. Similarly, ionomycin acts by elevating intracellular calcium levels, which activates calmodulin-dependent kinases capable of phosphorylating ZFP345. This mechanism is also shared by histamine, which influences intracellular calcium pathways activating PKC, which may phosphorylate ZFP345.
In addition to these, epidermal growth factor engages the MAPK/ERK pathway, which is another route through which ZFP345 can be phosphorylated. This pathway is crucial in the regulation of cell division and differentiation. Hydrogen peroxide, through the induction of oxidative stress, can activate various kinases, which may include those phosphorylating ZFP345. Calyculin A and okadaic acid take an alternative approach by inhibiting protein phosphatases, which would otherwise dephosphorylate proteins like ZFP345, leading to a net increase in its phosphorylated, active state. Anisomycin activates the JNK pathway, which then can target ZFP345 for phosphorylation. Ouabain, through its inhibition of Na⁺/K⁺-ATPase, alters ion gradients and activates signaling pathways that lead to ZFP345 phosphorylation. Lastly, 8-Br-cAMP and isoproterenol work by increasing cAMP levels, which activates PKA, leading to the phosphorylation of ZFP345, showcasing the diverse array of mechanisms through which ZFP345 can be activated by different chemical signals.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Activates protein kinase C (PKC) which in turn can activate ZFP345 by phosphorylation. PKC is known to phosphorylate a wide range of target proteins that are involved in various cellular functions. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Increases intracellular calcium levels, which activates calmodulin-dependent kinases (CaMK). These kinases can phosphorylate ZFP345, resulting in its activation. Calcium signaling plays a vital role in various cellular processes, including the activation of proteins through phosphorylation. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
Can create conditions that activate kinases capable of phosphorylating ZFP345. Oxidative stress can activate signaling pathways that lead to the activation of proteins through phosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Inhibits protein phosphatases, leading to increased phosphorylation and potential activation of ZFP345. Protein phosphatase inhibition can result in sustained activation of proteins by preventing dephosphorylation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Activates the JNK pathway, which can phosphorylate and activate ZFP345. The JNK pathway is involved in cellular responses to stress and is known to activate proteins through phosphorylation. | ||||||
Histamine, free base | 51-45-6 | sc-204000 sc-204000A sc-204000B | 1 g 5 g 25 g | $94.00 $283.00 $988.00 | 7 | |
Through its effects on intracellular calcium pathways, can activate protein kinase C (PKC), which may lead to phosphorylation and activation of ZFP345. Histamine receptors are involved in a variety of cellular signaling pathways, including those that activate kinases like PKC. | ||||||
Ouabain-d3 (Major) | sc-478417 | 1 mg | $516.00 | |||
Inhibition of Na⁺/K⁺-ATPase by Ouabain can alter ion balance and activate signal transduction pathways that may lead to the phosphorylation and activation of ZFP345. Na⁺/K⁺-ATPase activity is crucial for maintaining ion gradients that are essential for various cellular processes, including signal transduction. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
A cAMP analog that activates PKA, which can phosphorylate and activate ZFP345. This compound is used to mimic the effects of cAMP and activate cAMP-dependent pathways. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
Acts as an agonist at beta-adrenergic receptors, raising cAMP levels and leading to the activation of PKA, which can phosphorylate and activate ZFP345. Beta-adrenergic receptors are part of the sympathetic nervous system and can activate significant cellular signaling cascades. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Inhibits protein phosphatases, which leads to an increase in phosphorylated proteins and could lead to the activation of ZFP345. Okadaic Acid is known for its role in promoting phosphorylation through inhibition of phosphatases, which could sustain or enhance the activation state of proteins. | ||||||