WIRE Activators
WIRE Activators encompass a diverse array of chemical compounds that enhance the activity of WIRE through distinct and specific signaling pathways integral to cellular actin dynamics. Forskolin, by increasing cAMP, activates PKA, which can phosphorylate substrates impacting the actin polymerization role of WIRE. Similarly, PMA's role as a PKC activator leads to phosphorylation events that favor WIRE's interaction with actin, thus promoting actin polymerization. The EGCG compound, a known kinase inhibitor, may enhance WIRE's functionality by suppressing competitive kinases and thereby indirectly supporting WIRE's role in actin stabilization. Sphingosine-1-phosphate, through its receptor-mediated signaling, can potentiate the cytoskeletal reorganization where WIRE is a key player. The PI3K inhibitor LY294002, by altering the PI3K/AKT pathway, indirectly fosters an environment where WIRE's actin-binding activity is amplified. U73122's inhibitory effect on PLC can similarly alter downstream signaling, enhancing WIRE's role in the actin cytoskeleton regulation.
Further contributing to the activation of WIRE are Bisindolylmaleimide I, which, by inhibiting PKC, may enhance WIRE's engagement with actin polymerization processes, and Calyculin A, a phosphatase inhibitor that can lead to increased phosphorylation within pathways involving WIRE, thereby indirectly promoting its activity. Jasplakinolide's stabilization of actin filaments inherently supports WIRE's function by fostering firmer interactions with actin structures.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $76.00 $150.00 $725.00 $1385.00 $2050.00 | 73 | |
Forskolin directly elevates intracellular cyclic AMP (cAMP) levels, which activates protein kinase A (PKA). PKA phosphorylates various substrates that can lead to the activation of the WIRE protein, as PKA-mediated phosphorylation is known to regulate actin polymerization where WIRE is a crucial actin-binding component. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $40.00 $129.00 $210.00 $490.00 $929.00 | 119 | |
PMA functions as a potent protein kinase C (PKC) activator. PKC activation can result in the phosphorylation of downstream effectors that interact with WIRE, enhancing its ability to bind to actin and promote actin polymerization, thus increasing WIRE functional activity in cytoskeletal rearrangements. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $42.00 $72.00 $124.00 $238.00 $520.00 $1234.00 | 11 | |
EGCG acts as a kinase inhibitor, potentially reducing competitive signaling that may otherwise attenuate the pathways that WIRE is involved in. By inhibiting certain kinases, EGCG can indirectly foster an environment where WIRE's role in actin dynamics is enhanced due to less phosphorylation of competing substrates. | ||||||
D-erythro-Sphingosine-1-phosphate | 26993-30-6 | sc-201383 sc-201383D sc-201383A sc-201383B sc-201383C | 1 mg 2 mg 5 mg 10 mg 25 mg | $162.00 $316.00 $559.00 $889.00 $1693.00 | 7 | |
Sphingosine-1-phosphate engages with its receptors to activate signaling pathways that can lead to cytoskeletal reorganization. This lipid signaling molecule can potentiate the activity of WIRE by facilitating the actin polymerization processes, a key area where WIRE is known to function. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $121.00 $392.00 | 148 | |
LY294002 is a PI3K inhibitor that alters the PI3K/AKT pathway, which is involved in actin cytoskeleton organization. By modulating this pathway, LY294002 can create a signaling milieu that indirectly enhances the activity of WIRE in its role of actin binding and stabilization within cells. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $103.00 $237.00 | 36 | |
Bisindolylmaleimide I is a specific PKC inhibitor that can tilt the balance of cellular signaling towards pathways that enhance the activity of WIRE. By selectively inhibiting PKC, the compound may reduce the phosphorylation of proteins that compete with WIRE for actin binding, thus enhancing WIRE's role in actin polymerization. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $160.00 $750.00 | 59 | |
Calyculin A acts as a phosphatase inhibitor, specifically inhibiting PP1 and PP2A. The inhibition of these phosphatases can lead to increased phosphorylation levels of proteins within the pathways WIRE is involved in, which can indirectly enhance WIRE's activity in actin polymerization and stabilization. | ||||||
Jasplakinolide | 102396-24-7 | sc-202191 sc-202191A | 50 µg 100 µg | $180.00 $299.00 | 59 | |
Jasplakinolide stabilizes actin filaments and can induce actin polymerization. By stabilizing actin filaments, Jasplakinolide can enhance the functional activity of WIRE by promoting its interaction with stable actin structures, thereby playing a role in the dynamics of the cytoskeleton. | ||||||