SSB-4 Inhibitors
SSB-4 inhibitors are a class of chemical compounds that specifically target and inhibit the function of SSB-4, a protein involved in DNA replication, repair, and recombination processes. SSB-4, short for Single-Stranded DNA-Binding Protein 4, plays a critical role in stabilizing single-stranded DNA (ssDNA) during various phases of DNA metabolism, such as replication forks, DNA repair intermediates, and recombination events. By binding to ssDNA, SSB-4 prevents secondary structures from forming and protects the DNA strand from degradation, ensuring that these processes proceed efficiently. Inhibition of SSB-4 disrupts its ability to bind and stabilize ssDNA, potentially leading to errors in replication, ineffective repair mechanisms, or improper recombination events.
The mechanism of SSB-4 inhibitors typically involves interfering with the protein's ssDNA-binding domain, preventing it from attaching to and protecting single-stranded regions of the DNA. These inhibitors may alter the conformation of the protein, block its DNA-binding site, or disrupt its ability to interact with other proteins involved in DNA metabolism. Researchers use SSB-4 inhibitors to study the role of ssDNA-binding proteins in maintaining genome stability, particularly in understanding how they contribute to accurate replication and repair. By inhibiting SSB-4, scientists can explore how disruptions in DNA stabilization affect cellular processes such as cell division, genome maintenance, and response to DNA damage. These inhibitors are valuable tools for investigating the intricate mechanisms that ensure the fidelity of DNA processing and for unraveling the broader roles of single-stranded DNA-binding proteins in cellular homeostasis.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $176.00 $426.00 | 43 | |
Doxorubicin intercalates DNA, disrupting the replication process. This can downregulate SSB-4 expression by inhibiting the transcriptional machinery necessary for its gene expression. | ||||||
Actinomycin D | 50-76-0 | sc-200906 sc-200906A sc-200906B sc-200906C sc-200906D | 5 mg 25 mg 100 mg 1 g 10 g | $74.00 $243.00 $731.00 $2572.00 $21848.00 | 53 | |
Actinomycin D binds to DNA at the transcription initiation complex, preventing the elongation step of RNA synthesis, which could decrease SSB-4 mRNA synthesis and, consequently, its protein expression. | ||||||
Mitomycin C | 50-07-7 | sc-3514A sc-3514 sc-3514B | 2 mg 5 mg 10 mg | $66.00 $101.00 $143.00 | 85 | |
Mitomycin C forms DNA crosslinks, thereby hindering DNA replication and transcription. This activity can lead to the reduced expression of DNA repair proteins, including SSB-4. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $58.00 $186.00 $94.00 | 21 | |
Camptothecin stabilizes the DNA topoisomerase I complex, inducing DNA breaks and potentially reducing the expression of DNA repair proteins such as SSB-4 through DNA damage response pathways. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
Etoposide inhibits DNA topoisomerase II, leading to DNA breaks and the activation of DNA damage responses, which may include the repression of certain DNA repair protein genes, including that of SSB-4. | ||||||
Ellipticine | 519-23-3 | sc-200878 sc-200878A | 10 mg 50 mg | $145.00 $569.00 | 4 | |
Ellipticine intercalates into DNA and inhibits topoisomerase II, similar to Etoposide and Daunorubicin. This may lead to decreased expression of DNA repair proteins including SSB-4. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $138.00 $380.00 | 101 | |
Cisplatin forms DNA crosslinks that inhibit transcription and replication. This can lead to a decreased expression of DNA repair proteins such as SSB-4. | ||||||
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $78.00 $260.00 | 18 | |
Hydroxyurea inhibits ribonucleotide reductase, reducing deoxyribonucleotide pools and arresting DNA synthesis, which may lead to downregulation of SSB-4 expression due to reduced replication demand. | ||||||
Triptolide | 38748-32-2 | sc-200122 sc-200122A | 1 mg 5 mg | $90.00 $204.00 | 13 | |
Triptolide has been shown to inhibit the transcription of various genes, which potentially includes the gene encoding SSB-4, leading to decreased protein levels. | ||||||
Chlorambucil | 305-03-3 | sc-204682 sc-204682A | 250 mg 1 g | $52.00 $122.00 | 3 | |
Chlorambucil alkylates DNA, leading to strand breaks and potentially reduced expression of DNA repair proteins such as SSB-4 due to engagement of DNA damage checkpoints. | ||||||