Ska2 Activators
The Spindle and Kinetochore Associated Complex Subunit 2 (Ska2) protein is an integral component of the Ska complex, playing a pivotal role in the regulation of chromosome segregation during mitosis. This protein, along with its counterparts in the Ska complex, facilitates the proper attachment of chromosomes to the mitotic spindle, ensuring accurate chromosome alignment and segregation. This mechanism is vital for maintaining genomic stability, as it prevents aneuploidy and supports the faithful transmission of genetic information to daughter cells. Ska2's function is critical during the metaphase to anaphase transition, where it contributes to the generation of sufficient tension across sister chromatids, signaling that all chromosomes are correctly bi-oriented and allowing for the onset of anaphase. The precise control of this process is essential for cell viability and proliferation, highlighting the importance of Ska2 in cellular dynamics and its role in the safeguarding of genomic integrity.
The activation of Ska2 within the Ska complex involves a series of tightly regulated steps that are crucial for its role in mitosis. This activation is primarily governed by post-translational modifications and interactions with other mitotic regulators. Phosphorylation plays a key role in modulating the activity and localization of Ska2, with specific kinases targeting Ska2 at different stages of mitosis to ensure its timely function. For example, the activation of Ska2 can be facilitated by the cyclin-dependent kinases (Cdks) in coordination with other mitotic kinases, which phosphorylate Ska2, enhancing its affinity for kinetochores and stabilizing its association with the mitotic spindle. Furthermore, the interaction between Ska2 and other components of the Ska complex is essential for its activation, as these interactions are necessary for the complex's localization to kinetochores and its function in tension sensing and signal transduction.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
L-α-Lecithin, Egg Yolk, Highly Purified | 8002-43-5 | sc-203096 | 250 mg | $135.00 | ||
L-α-Lecithin on the inner leaflet of the plasma membrane can be recognized by Ska2 as part of the Ska complex, aiding in proper attachment to the mitotic spindle during cell division, thereby enhancing Ska2's role in chromosome segregation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A is a potent inhibitor of protein phosphatases 1 and 2A, which prevents dephosphorylation of proteins that may interact with Ska2, thus indirectly maintaining Ska2 in an active state necessary for spindle checkpoint functions. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Like Calyculin A, Okadaic Acid is a selective inhibitor of protein phosphatases. This action preserves the phosphorylation state of Ska2-interacting partners, indirectly enhancing Ska2's function in the spindle assembly checkpoint. | ||||||
Taxol | 33069-62-4 | sc-201439D sc-201439 sc-201439A sc-201439E sc-201439B sc-201439C | 1 mg 5 mg 25 mg 100 mg 250 mg 1 g | $41.00 $74.00 $221.00 $247.00 $738.00 $1220.00 | 39 | |
Paclitaxel stabilizes microtubules and can enhance Ska2's interactions with stabilized spindle microtubules, indirectly promoting Ska2's activity in chromosome alignment and segregation. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $60.00 $265.00 $1000.00 | 163 | |
MG132 is a proteasome inhibitor that can indirectly increase the stability of Ska2 by preventing its degradation, potentially enhancing the protein's activity in mitosis. | ||||||
ZM-447439 | 331771-20-1 | sc-200696 sc-200696A | 1 mg 10 mg | $153.00 $356.00 | 15 | |
ZM447439 is an Aurora kinase inhibitor that can potentially increase the importance of Ska2's role in the spindle checkpoint by altering the phosphorylation dynamics of proteins involved in kinetochore-microtubule attachment. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I is a specific inhibitor of protein kinase C, which may lead to altered phosphorylation states of proteins that interact with Ska2, potentially enhancing its role in the spindle assembly checkpoint. | ||||||