Scc1 Activators
Scc1 activators encompass a range of chemical compounds that exert their effects through various biochemical pathways, ultimately enhancing the functional activity of Scc1 in sister chromatid cohesion. Forskolin, through its ability to raise intracellular cAMP levels, indirectly augments Scc1's activity by PKA-mediated phosphorylation of cohesin complex proteins or associated regulators. Similarly, PMA, which activates PKC, and Bisindolylmaleimide I, a PKC inhibitor, can modulate phosphorylation events that indirectly enhance Scc1's role in maintaining sister chromatid cohesion. Okadaic acid and calyculin A, by inhibiting protein phosphatases PP1 and PP2A, increase the phosphorylation of cohesin complex members, which may enhance the stability and function of Scc1. Ionomycin and thapsigargin elevate intracellular calcium levels, potentially activating calcium-dependent protein kinases and thus influencing Scc1 activity.
Scc1 activators are a diverse array of chemical compounds that indirectly enhance the functional activity of Scc1 through modulation of cellular signaling pathways and phosphorylation states. Forskolin, which stimulates adenylyl cyclase to raise intracellular cAMP levels, activates protein kinase A (PKA), potentially leading to phosphorylation events that augment the role of Scc1 in sister chromatid cohesion. Phorbol 12-myristate 13-acetate (PMA) capitalizes on the activation of protein kinase C (PKC) to indirectly influence the phosphorylation state of cohesin complex proteins, thereby enhancing Scc1's function. The inhibition of protein phosphatases PP1 and PP2A by okadaic acid and calyculin A, respectively, prevents dephosphorylation of proteins within the cohesin complex, possibly resulting in improved Scc1 activity. Epigallocatechin gallate (EGCG) and lithium chloride interact with various kinases and phosphatases, leading to modified phosphorylation dynamics that could indirectly promote Scc1's role in chromosomal segregation.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin directly stimulates adenylyl cyclase, increasing cAMP levels in the cell. This elevation in cAMP may lead to the activation of PKA, which could then phosphorylate proteins that interact with or regulate Scc1, thus potentially enhancing its function in sister chromatid cohesion. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is involved in a multitude of cellular processes. PKC activation could enhance Scc1 function by phosphorylation of cohesin complex proteins or regulatory factors involved in chromatid cohesion. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
This compound is a potent inhibitor of protein phosphatases PP1 and PP2A. The inhibition of these phosphatases could lead to increased phosphorylation levels of proteins within the cohesin complex, indirectly enhancing Scc1's role in sister chromatid cohesion. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to okadaic acid, calyculin A inhibits protein phosphatases such as PP1 and PP2A, potentially increasing the phosphorylation state of cohesin complex proteins, which may enhance the function of Scc1. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
EGCG can modulate various signaling pathways and may indirectly influence the activity of protein kinases and phosphatases, which can alter the phosphorylation status of Scc1 or its complex partners, potentially enhancing cohesin function. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can activate calmodulin-dependent kinase II (CaMKII), which may indirectly enhance the phosphorylation of proteins involved with Scc1 function. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to increased cytosolic calcium levels. This may activate calcium-dependent protein kinases that could phosphorylate and enhance the function of Scc1. | ||||||
Staurosporine | 62996-74-1 | sc-3510 sc-3510A sc-3510B | 100 µg 1 mg 5 mg | $82.00 $153.00 $396.00 | 113 | |
Staurosporine is a broad-spectrum protein kinase inhibitor, but at low concentrations, it can selectively activate certain kinases. This could lead to selective phosphorylation events that enhance Scc1 activity. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
As a specific inhibitor of PKC, Bisindolylmaleimide I could lead to compensatory activation of other kinases that may enhance the phosphorylation and activity of Scc1, by relieving negative feedback loops. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $108.00 $169.00 $295.00 $561.00 $835.00 | 2 | |
This cAMP analog can activate PKA, leading to phosphorylation events that could enhance the activity of Scc1 by modifying its interaction with other cohesin complex proteins or regulatory factors. | ||||||