Rub1 Inhibitors
Chemical inhibitors of Rub1 function by disrupting various steps in the post-translational modification processes in which Rub1 is involved. MLN4924, also known as Pevonedistat, directly inhibits the NEDD8-activating enzyme. This enzyme is crucial for the neddylation process, where Rub1 is conjugated to its target proteins. By blocking this activation step, the functional role of Rub1 in post-translational modifications is impaired. Similarly, MLN7243, or TAK-243, targets the ubiquitin-activating enzyme (UAE), which affects the ubiquitin-proteasome system. Although UAE is primarily associated with ubiquitin, its inhibition can disrupt the recycling and function of ubiquitin-like modifiers such as Rub1. PYR-41 is another inhibitor that targets the ubiquitin-activating enzyme E1, impairing the activation and subsequent conjugation of ubiquitin-like proteins including Rub1.
Moreover, IU1 specifically inhibits the deubiquitinating enzyme USP14, which, despite its primary role in targeting ubiquitin, can also influence the stability and function of Rub1-modified proteins. This is due to its effect on the proteasome system, which is involved in the degradation and recycling of ubiquitin and ubiquitin-like proteins. In a similar vein, b-AP15 and Clasto-lactacystin β-lactone inhibit the deubiquitinating activity and the proteasome itself, respectively. These inhibitors can lead to an accumulation of Rub1-conjugated proteins by preventing their degradation and disrupting the normal turnover and function of Rub1.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
MLN 4924 | 905579-51-3 | sc-484814 | 1 mg | $280.00 | 1 | |
MLN4924 inhibits the NEDD8-activating enzyme (NAE), which is crucial for the neddylation process. Rub1, the yeast homolog of NEDD8, is involved in neddylation, and MLN4924's inhibition of NAE prevents Rub1 from conjugating to its substrates, leading to functional inhibition of Rub1-related neddylation. | ||||||
Ubiquitin E1 Inhibitor, PYR-41 | 418805-02-4 | sc-358737 | 25 mg | $360.00 | 4 | |
PYR-41 is an inhibitor of the ubiquitin-activating enzyme E1. By inhibiting E1, PYR-41 indirectly prevents the activation of ubiquitin-like proteins including Rub1, thereby inhibiting their conjugation to target proteins and subsequent downstream signaling pathways that rely on Rub1-dependent modifications. | ||||||
IU1 | 314245-33-5 | sc-361215 sc-361215A sc-361215B | 10 mg 50 mg 100 mg | $138.00 $607.00 $866.00 | 2 | |
IU1 specifically inhibits the deubiquitinating enzyme USP14. While USP14 primarily targets ubiquitin, its inhibition can also affect ubiquitin-like proteins, including Rub1. The inhibition of deubiquitinase activity can lead to altered degradation and signaling pathways involving Rub1-modified proteins due to the disruption of the proteasome system. | ||||||
Lactacystin | 133343-34-7 | sc-3575 sc-3575A | 200 µg 1 mg | $165.00 $575.00 | 60 | |
Clasto-lactacystin β-lactone irreversibly inhibits the proteasome. This action can result in increased levels of Rub1-conjugated proteins due to decreased degradation, indirectly inhibiting the recycling and proper function of Rub1 within its associated pathways. | ||||||
Gö 6983 | 133053-19-7 | sc-203432 sc-203432A sc-203432B | 1 mg 5 mg 10 mg | $103.00 $293.00 $465.00 | 15 | |
G5 inhibits the ubiquitin E1 enzyme, and by doing so, it can indirectly affect Rub1 by disrupting the balance and recycling of ubiquitin-like modifiers, which may lead to a functional inhibition of Rub1 conjugation processes and its downstream pathways. | ||||||
WP1130 | 856243-80-6 | sc-364650 sc-364650A | 10 mg 50 mg | $480.00 $1455.00 | 1 | |
WP1130 selectively inhibits deubiquitinases, leading to an accumulation of ubiquitinated proteins. As with ubiquitination, neddylation processes involving Rub1 could be indirectly affected, as the perturbation of deubiquitination may influence Rub1's role within its neddylation pathways. | ||||||
M 344 | 251456-60-7 | sc-203124 sc-203124A | 1 mg 5 mg | $107.00 $316.00 | 8 | |
NSC 687852, also known as Ubistatin A, inhibits the ubiquitin-proteasome pathway. While it mainly impacts ubiquitination, the disruption can indirectly inhibit the function of Rub1 by altering the cellular context within which Rub1 operates, potentially affecting its conjugation and substrate interactions. | ||||||