RNF186 Inhibitors
The inhibitors of RNF186 constitute a chemically diverse assemblage, each distinguished by unique mechanisms of action that collectively exert regulatory influence on RNF186 within cellular contexts. RNF186, a Ring Finger Protein (RFP) E3 ubiquitin ligase, plays a pivotal role in ubiquitin-mediated protein degradation and gene expression regulation. The strategic modulation of RNF186 activity is crucial for maintaining cellular homeostasis and responding to various intracellular signals. MLN4924 and Pevonedistat exert their effects by disrupting cullin neddylation, an essential post-translational modification crucial for the activation of Cullin-RING E3 ligases, a group to which RNF186 belongs. This indirect modulation influences RNF186 function by altering the activity of these ligases, thereby impacting the ubiquitination and subsequent degradation of specific target proteins. Nutlin-3 and MLN7243, through interference with the p53-MDM2 interaction, indirectly influence RNF186-mediated ubiquitination of p53, a central player in cellular stress responses.
Bortezomib, MG-132, and CB-5083 act as inhibitors of the 26S proteasome, a key cellular machinery responsible for protein degradation. The impact of these inhibitors on protein turnover dynamics directly modulates RNF186-regulated protein degradation pathways, shaping the cellular protein landscape. NSC697923, by inhibiting the E1 ubiquitin-activating enzyme, disrupts ubiquitin conjugation processes, thereby affecting the regulatory role of RNF186 in ubiquitin-mediated protein turnover. A485 and C646, selective inhibitors of p300/CBP, indirectly modulate RNF186-mediated signaling pathways associated with gene expression regulation, highlighting the intricate interplay between RNF186 and chromatin-modifying enzymes. This diverse repertoire of inhibitors serves as an invaluable toolkit for researchers, affording them the means to meticulously investigate the intricate cellular pathways orchestrating the regulation of RNF186 and its multifaceted roles in ubiquitin-mediated protein degradation and gene expression. The comprehensive elucidation of these chemical interactions significantly contributes to the advancement of our understanding of RNF186 regulation in diverse cellular contexts, shedding light on the intricate molecular mechanisms governing cellular homeostasis.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
MLN 4924 | 905579-51-3 | sc-484814 | 1 mg | $280.00 | 1 | |
MLN4924 inhibits NEDD8-activating enzyme (NAE), preventing cullin neddylation in the ubiquitin-proteasome system. By disrupting this pathway, it indirectly modulates RNF186. Cullin neddylation is crucial for the activity of Cullin-RING E3 ligases, and RNF186, being an E3 ligase, is influenced by perturbations in this ubiquitin-proteasome system, resulting in altered protein turnover and potentially impacting cellular processes regulated by RNF186. | ||||||
Nutlin-3 | 548472-68-0 | sc-45061 sc-45061A sc-45061B | 1 mg 5 mg 25 mg | $56.00 $212.00 $764.00 | 24 | |
Nutlin-3 disrupts the interaction between p53 and MDM2, preventing p53 degradation. As RNF186 is implicated in regulating p53 stability, the inhibition of MDM2-p53 interaction indirectly influences RNF186-mediated ubiquitination of p53. This disruption leads to the stabilization of p53, altering the cellular landscape and indirectly impacting RNF186's role in controlling protein turnover and modulating downstream cellular responses associated with its regulatory functions. | ||||||
MLN7243 | 1450833-55-2 | sc-507338 | 5 mg | $340.00 | ||
MLN7243 is a peptidomimetic inhibitor of the E3 ligase activity of HDM2, disrupting ubiquitination pathways associated with p53. As RNF186 has interactions with p53, inhibition of HDM2 indirectly modulates RNF186-mediated ubiquitination events. The altered ubiquitin-proteasome system can impact RNF186's regulatory role in protein turnover, influencing the stability and function of target proteins, ultimately shaping cellular responses governed by RNF186. | ||||||
Bortezomib | 179324-69-7 | sc-217785 sc-217785A | 2.5 mg 25 mg | $132.00 $1064.00 | 115 | |
Bortezomib inhibits the 26S proteasome, disrupting protein degradation pathways. This indirectly influences RNF186 by altering the ubiquitin-proteasome system, impacting protein turnover. The perturbation in protein degradation dynamics can lead to changes in the stability and abundance of substrates targeted by RNF186, consequently modulating its regulatory functions in cellular processes associated with protein homeostasis and ubiquitin-mediated protein degradation. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG-132 inhibits the 26S proteasome, disrupting protein degradation pathways. This indirectly influences RNF186 by altering the ubiquitin-proteasome system, impacting protein turnover. The perturbation in protein degradation dynamics can lead to changes in the stability and abundance of substrates targeted by RNF186, consequently modulating its regulatory functions in cellular processes associated with protein homeostasis and ubiquitin-mediated protein degradation. | ||||||
NSC697923 | 343351-67-7 | sc-391107 sc-391107A | 1 mg 5 mg | $15.00 $51.00 | 3 | |
NSC697923 inhibits the E1 ubiquitin-activating enzyme, disrupting ubiquitin conjugation pathways. By interfering with the ubiquitin-proteasome system, it indirectly modulates RNF186. The altered ubiquitin conjugation dynamics influence protein turnover, affecting the stability and abundance of substrates targeted by RNF186. | ||||||
C646 | 328968-36-1 | sc-364452 sc-364452A | 10 mg 50 mg | $260.00 $925.00 | 5 | |
C646 is a selective inhibitor of the histone acetyltransferase p300/CBP. As RNF186 interacts with p300/CBP, inhibiting their activity indirectly influences RNF186-mediated signaling pathways. The disruption in histone acetylation dynamics alters the cellular landscape, indirectly impacting RNF186's role in controlling gene expression and modulating downstream cellular responses associated with its regulatory functions. | ||||||