RISC Activators
RISC activators, also known as RNA-induced silencing complex activators, represent a fascinating class of molecules in the realm of molecular biology and genetic regulation. These activators play a crucial role in the intricate machinery of RNA interference (RNAi), a fundamental biological process that allows cells to control gene expression post-transcriptionally. RNAi is a highly conserved mechanism found in a wide range of organisms, from plants to animals, and it involves the suppression of gene expression by selectively degrading or inhibiting the translation of messenger RNAs (mRNAs). At the heart of the RNAi pathway lies the RNA-induced silencing complex (RISC), and RISC activators are key players that modulate its activity.
RISC activators are molecules that facilitate the assembly and activation of the RISC complex. The RISC complex consists of small RNA molecules, such as small interfering RNAs (siRNAs) or microRNAs (miRNAs), bound to Argonaute proteins. These small RNAs serve as guides, directing the RISC complex to specific target mRNAs that share sequence complementarity with the small RNA. When a RISC activator is present, it aids in the loading of the small RNA onto the Argonaute protein within the RISC complex, ensuring efficient and precise target recognition. Additionally, RISC activators may enhance the catalytic activity of the RISC complex, further promoting the degradation of target mRNAs or inhibiting their translation.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Spermidine | 124-20-9 | sc-215900 sc-215900B sc-215900A | 1 g 25 g 5 g | $57.00 $607.00 $176.00 | ||
Spermidine promotes the activity of RISC by facilitating the binding of short RNAs to the complex, which is essential for RISC's gene-silencing function. This polyamine can stabilize the RNA-induced silencing complex structure, enhancing its affinity for the short RNA molecules that guide the complex to silence gene expression. | ||||||
Magnesium chloride | 7786-30-3 | sc-255260C sc-255260B sc-255260 sc-255260A | 10 g 25 g 100 g 500 g | $28.00 $35.00 $48.00 $125.00 | 2 | |
Magnesium ions are crucial for the RNA cleavage activity of RISC. They stabilize the tertiary structure of the RNA duplexes within the complex and act as cofactors for the endonucleolytic activity of Argonaute proteins, which are core components of RISC. | ||||||
Potassium Chloride | 7447-40-7 | sc-203207 sc-203207A sc-203207B sc-203207C | 500 g 2 kg 5 kg 10 kg | $55.00 $155.00 $285.00 $455.00 | 5 | |
Potassium chloride affects the electrostatic environment of RISC, improving its interaction with RNA molecules. The presence of potassium ions can enhance the conformational stability of RISC, facilitating its RNA silencing activity. | ||||||
ADP | 58-64-0 | sc-507362 | 5 g | $54.00 | ||
Adenosine triphosphate (ATP) provides the energy required for the helicase activity associated with RISC loading of the RNA duplex. This energy is necessary for unwinding the RNA strands and allows the RISC to incorporate the single-stranded RNA that guides the complex to its target mRNAs. | ||||||
Guanosine-5′-Triphosphate, Disodium salt | 86-01-1 | sc-507564 | 1 g | $714.00 | ||
Guanosine triphosphate (GTP) is utilized by RISC as a substrate for the GTPase activity associated with some of its protein components. The hydrolysis of GTP to GDP provides energy for structural changes in the RISC complex that enhance its ability to bind and process RNA molecules. | ||||||
Cacodylic acid | 75-60-5 | sc-239453 sc-239453A sc-239453B | 5 g 10 g 25 g | $60.00 $103.00 $204.00 | ||
Cacodylic acid buffers the pH and maintains the stability of RISC in vitro, which is essential for its optimal activity. The buffer can enhance RISC's RNAi activity by preserving the structural integrity of the complex in the reaction conditions. | ||||||
Manganese(II) chloride beads | 7773-01-5 | sc-252989 sc-252989A | 100 g 500 g | $19.00 $31.00 | ||
Manganese chloride is a cofactor that can enhance the catalytic activity of the Argonaute proteins within RISC. Manganese ions can also affect the conformation of RNA, potentially improving the interaction between RISC and its RNA substrates. | ||||||
Tris, Hydrochloride | 1185-53-1 | sc-216106 sc-216106A sc-216106B | 500 g 1 kg 25 kg | $195.00 $317.00 $4244.00 | 1 | |
Tris-HCl buffer ensures the optimal pH for RISC activity, enhancing its RNA interference efficiency. The buffer can protect RISC components from denaturation and maintain the conformational flexibility necessary for RNA binding and cleavage. | ||||||
β-Mercaptoethanol | 60-24-2 | sc-202966A sc-202966 | 100 ml 250 ml | $90.00 $120.00 | 10 | |
β-Mercaptoethanol serves as a reducing agent similar to DTT, preserving the active site conformation of Argonaute proteins in RISC. By preventing oxidative damage, it maintains the structural integrity of RISC, thus enhancing its ability to guide RNA-induced gene silencing. | ||||||
Tween-20 | 9005-64-5 | sc-29113C sc-29113 sc-29113B sc-29113A | 100 ml 500 ml 1 L 3.8 L | $19.00 $31.00 $51.00 $163.00 | 54 | |
Tween-20 is a non-ionic detergent that can be used at low concentrations to stabilize RISC by preventing non-specific aggregation of its protein components. By maintaining the solubility of RISC, Tween-20 ensures the complex remains active and capable of binding RNA. | ||||||