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Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. Tris, Hydrochloride has also been used with urea as a method for antigen retrieval in immunohistochemistry. In smooth muscles Tris, Hydrochloride has been observed to inhibit motor responses to adrenergic motor nerve stimulation. In the crystalization of porcine pancreatic elastase with use of Tris, Hydrochloride, the Tris, Hydrochloride buffer was observed to induce conformational change and crystal-packing contraction. Tris, Hydrochloride has also been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs.
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