Date published: 2025-10-15

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Tween-20 (CAS 9005-64-5)

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Alternate Names:
Tween-20 is also known as Polysorbate 20.
Application:
Tween-20 is a membrane protein solubilizing, hydrophilic, non-ionic surfactant and polyoxyethylene derivative of sorbitan monolaurate for nanomaterial synthesis.
CAS Number:
9005-64-5
Molecular Weight:
1227.54
Molecular Formula:
C58H114O26
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Tween-20 is a hydrophilic, non-ionic detergent surfactant that is suitable for the isolation of water-soluble membrane proteins. It is used in cosmetic and personal care product formulations as a fragrance ingredient, surfactant, emulsifier, and wetting agent and is employed in a variety of products including skin fresheners, skin care products, skin cleansing products, makeup bases and foundations, shampoos, permanent waves and fragrance powders. Tween-20 is recommended for ELISA and immunoblots. It is used as an additive in food, pharmaceuticals and cosmetics. Tween-20 is also known as Polyethylene glycol sorbitan monolaurate, Polysorbate 20, Sorbimacrogol Laurate 300 and Polyoxyethylenesorbitan monolaurate.


Tween-20 (CAS 9005-64-5) References

  1. Screening of endocrine disrupting chemicals with MELN cells, an ER-transactivation assay combined with cytotoxicity assessment.  |  Berckmans, P., et al. 2007. Toxicol In Vitro. 21: 1262-7. PMID: 17572059
  2. Development of an in vitro corrosion/irritation prediction assay using the EpiDerm skin model.  |  Kidd, DA., et al. 2007. Toxicol In Vitro. 21: 1292-7. PMID: 17936572
  3. Factors influencing the partitioning and toxicity of nanotubes in the aquatic environment.  |  Kennedy, AJ., et al. 2008. Environ Toxicol Chem. 27: 1932-41. PMID: 19086318
  4. Flow cytometry analysis of transcription factors in T lymphocytes.  |  Albu, DI., et al. 2010. Methods Mol Biol. 647: 377-90. PMID: 20694680
  5. Quantification of unadsorbed protein and surfactant emulsifiers in oil-in-water emulsions.  |  Berton, C., et al. 2011. J Colloid Interface Sci. 354: 739-48. PMID: 21167495
  6. Penetration of antimicrobials to pulmonary epithelial lining fluid and muscle and impact of drug physicochemical properties determined by microdialysis.  |  Rottbøll, LA. and Friis, C. 2016. J Pharmacol Toxicol Methods. 78: 58-65. PMID: 26645525
  7. A modified microemulsion method for fabrication of hydrogel Tragacanth nanofibers.  |  Ghayempour, S. and Montazer, M. 2018. Int J Biol Macromol. 115: 317-323. PMID: 29649534
  8. Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli.  |  Black, PN., et al. 1987. J Biol Chem. 262: 1412-9. PMID: 3027089
  9. Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle.  |  Zhang, YR., et al. 2020. J Vis Exp.. PMID: 32065162
  10. Emulsifying and Anti-Oxidative Properties of Proteins Extracted from Industrially Cold-Pressed Rapeseed Press-Cake.  |  Östbring, K., et al. 2020. Foods. 9: PMID: 32466177
  11. Regulating droplet impact and wetting behaviors on hydrophobic leaves using a nonionic surfactant.  |  Zhao, R., et al. 2023. J Colloid Interface Sci. 629: 926-937. PMID: 36152617
  12. Immunofluorescence and Aggresome Staining of Nothobranchius furzeri Cryosections.  |  Bagnoli, S., et al. 2023. Cold Spring Harb Protoc.. PMID: 36878645
  13. Different blocking agents cause variation in the immunologic detection of proteins transferred to nitrocellulose membranes.  |  Spinola, SM. and Cannon, JG. 1985. J Immunol Methods. 81: 161-5. PMID: 3926901
  14. Immunoblotting and dot immunobinding--current status and outlook.  |  Towbin, H. and Gordon, J. 1984. J Immunol Methods. 72: 313-40. PMID: 6206159
  15. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes.  |  Batteiger, B., et al. 1982. J Immunol Methods. 55: 297-307. PMID: 6820029

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Tween-20, 100 ml

sc-29113C
100 ml
$18.00

Tween-20, 500 ml

sc-29113
500 ml
$30.00

Tween-20, 1 L

sc-29113B
1 L
$50.00

Tween-20, 3.8 L

sc-29113A
3.8 L
$160.00

I have a question regarding the tween-20 expiration. I prepared the 10% Tween-20 solution in DDW a year ago. I keep it in bench at room temperature, without avoiding light. Do you think there is a great chance of Tween to expire? Thank you !

Asked by: Fan Wenpeng
Thank you for your question. Please contact our technical service team directly and provide the lot number of your product.
Answered by: Tech Support Europe
Date published: 2025-02-13

Hi, What grade is your product sc-29113, Tween-20? (i.e. reagent, lab, food, cosmetic)

Asked by: Alex1698
Thank you for questions. SC-29113 is research grade only.
Answered by: Technical Service
Date published: 2022-04-23

Is sc-29113 a 100% Tween-20 nonionic detergent or is this 10% T20 solution? Thank you!

Asked by: BeaRoma
Yes, this is 100% Tween-20 and not a diluted solution.
Answered by: Technical Support
Date published: 2022-06-27

Is sc-29113, Tween-20 reagent grade or molecular grade?

Asked by: Jz28sail
Thank you for your question. The compound, sc-29113, is a high-quality reagent not marketed as a specific grade, nor do we purchase a specific grade. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-26
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Rated 5 out of 5 by from Great productWe use this product for many years especially as a detergent for TBST used for western blots and buffers used for immunohistofluorescence.
Date published: 2022-07-15
Rated 5 out of 5 by from 挺好用的我已经第二次购买sc-29113C了,挺好用的。附带的GFP抗体还在使用,不知道结果。但前期附带的Ubiquitin (P4D1)和Ubiquitin (P4G7)都挺好用的。
Date published: 2021-07-19
Rated 5 out of 5 by from 效果出众我两周前买了试剂,商家送来了两种抗体小样,效果都挺好的,特别是抗体,效价很高,不错不错不错。。。。。。
Date published: 2019-02-18
Rated 5 out of 5 by from Use it routinely in WBUse it routinely in WB, works good, no complaints
Date published: 2015-04-10
Rated 5 out of 5 by from SiddiquiSiddiqui, et. al. (PubMed ID 19393114) used product sc-29113 in wash and incubation buffers in WB analysis. -SCBT Publication Review
Date published: 2015-04-10
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Tween-20 is rated 5.0 out of 5 by 5.
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