Popular Stains, Dyes and Fluorescent Probes

Evans Blue is a synthetic dye characterized by its strong affinity for albumin, allowing it to selectively bind to plasma proteins. This property enables it to serve as a reliable tracer in various experimental settings. Its distinct spectral properties, including a sharp absorption peak, facilitate its use in fluorescence applications. Additionally, Evans Blue exhibits unique interactions with cellular membranes, enhancing its utility in studying permeability and transport mechanisms in biological systems.

Neutral Red Solution is a dynamic staining agent that exhibits strong affinity for acidic cellular compartments, particularly lysosomes. Its unique ability to shift in fluorescence intensity based on environmental pH makes it an excellent probe for studying cellular dynamics. The dye's interaction with nucleic acids and proteins allows for enhanced contrast in microscopy, facilitating detailed observation of cellular morphology and function. Its rapid uptake and retention in living cells further underscore its utility in biological research.

BCECF/AM is a versatile fluorescent probe known for its exceptional sensitivity to pH changes within cellular environments. Once inside the cell, it undergoes hydrolysis to release BCECF, which exhibits distinct fluorescence properties that vary with pH levels. This enables researchers to monitor intracellular pH dynamics in real-time. Its ability to interact with various cellular components makes it a powerful tool for studying metabolic processes and ion transport mechanisms.

4-Methylumbelliferyl α-L-Idopyranosiduronic Acid 2-Sulfate Disodium Salt is a highly effective fluorescent probe known for its exceptional sensitivity and specificity in detecting glycosidic bonds. Its unique structure allows for selective hydrolysis by glycosidases, resulting in a pronounced fluorescence increase. This compound exhibits rapid reaction kinetics, making it suitable for real-time monitoring of enzymatic activity. Its distinct spectral characteristics enable precise visualization in various analytical applications.

Acridine Orange Stain is a fluorescent dye known for its ability to intercalate into nucleic acids, allowing for the selective visualization of DNA and RNA within cells. Its unique properties enable it to emit distinct fluorescence under specific wavelengths, facilitating the differentiation of live and dead cells. The dye's affinity for acidic environments enhances its binding efficiency, making it a powerful tool for studying cellular morphology and dynamics in various biological contexts.

Anthrone is a versatile fluorescent probe known for its ability to interact with carbohydrates, particularly in the detection of sugars. Its unique structure allows for strong π-π stacking interactions, enhancing fluorescence intensity upon binding. This property makes it an effective tool for quantifying polysaccharides in various samples. Additionally, its solubility in organic solvents and stability under diverse conditions contribute to its widespread application in analytical chemistry and biochemistry.

Phalloidin-tetramethylrhodamine B isothiocyanate is a potent fluorescent probe that specifically binds to F-actin, a key component of the cytoskeleton. This selective affinity allows for detailed visualization of cellular architecture and dynamics. The compound exhibits high photostability and brightness, making it ideal for live-cell imaging. Its unique conjugation with tetramethylrhodamine enhances fluorescence intensity, facilitating the study of actin filament organization and cellular motility.

Hoechst 33342 trihydrochloride is a fluorescent dye renowned for its ability to selectively bind to DNA, particularly in regions rich in adenine-thymine pairs. This selective affinity allows for enhanced visualization of cellular nuclei under fluorescence microscopy. The dye exhibits a unique mechanism of intercalation, where it inserts between base pairs, resulting in a significant increase in fluorescence upon binding. Its high quantum yield and photostability make it ideal for long-term imaging applications.

MeOSuc-AAPV-AMC is a highly effective fluorescent probe characterized by its unique ability to undergo specific molecular interactions that enhance its luminescence. This compound features a tailored structure that promotes selective binding to target biomolecules, facilitating the visualization of cellular processes. Its rapid reaction kinetics allow for dynamic tracking of molecular events, while its distinct fluorescence properties enable sensitive detection in diverse experimental conditions, making it a powerful tool for probing biological mechanisms.

Astra Blue is a vibrant fluorescent dye characterized by its strong affinity for nucleic acids, making it an effective probe for visualizing genetic material. Its unique molecular structure allows for intercalation between base pairs, resulting in enhanced fluorescence upon binding. This property facilitates the detection of DNA and RNA in complex mixtures, while its stability under various conditions ensures reliable performance in diverse experimental setups. The dye's distinct emission profile further aids in multiplexing applications.