BCECF/AM CAS: 117464-70-7
MF: mixture of C30H20O11, C35H28O15 and C40H36O19
MW: Average = 688.59

BCECF/AM (CAS 117464-70-7)

BCECF/AM | CAS 117464-70-7 is rated 5.0 out of 5 by 1.
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Alternate Names: BCECF/AM Also known as 3′,6′-Bis(acetyloxy)-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-bis[(acetyloxy)methyl] Ester Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-dipropanoic Acid; SPIRO(ISOBENZOFURAN-1(3H),9′-(9H)XANTHENE)-2′,7′-DIPROPANOIC ACID
Application: BCECF/AM is a readily membrane-permeable fluorescent indicator for the measurement of cytoplasmic pH
CAS Number: 117464-70-7
Purity: ≥90%
Molecular Weight: Average = 688.59
Molecular Formula: mixture of C30H20O11, C35H28O15 and C40H36O19
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

BCECF/AM, a membrane-permeable fluorescent indicator for the measurement of cytoplasmic pH with an Abs/Em = 503/520 nm. BCECF/AM readily and rapidly diffuses through cell membranes. BCECF/AM is an acetoxymethyl ester of BCECF, is a variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. BCECF/AM is a selective vacuolar accumulation of the fluorescent pH-indicator used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.


References

1. Weiner, I.D., et al. 1989. Am. J. Physiol. 256: F957-F964. PMID: 2719125
2. Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163: 500-505. PMID: 2775282
3. Hayashi, H., et al. 1992. Am. J. Physiol. 262: C628-C634. PMID: 1550207
4. Sasaki, S., et al. 1992. Biochim. Biophys. Acta. 1137: 45-51. PMID: 1327152
5. Futsaether, C.M., et al. 1993. Can. J. Microbiol. 39: 180-186. PMID: 8467419

Usage :
Cell staining for detecting cytoplasmic pH.

• Suspend cells in HEPES buffer solution (sc-300789) to prepare 4x107 cells/ml.

• Add 1 mM BCECF-AM/DMSO solution to the cell suspension to prepare 3 uM BCECF-AM (1/300 vol of cell suspension) as the final concentration.

• Incubate the cell suspension at 37° C for 30 min.

• Wash the cells 3 times with HEPES buffer saline, and then prepare 3x106 cells/ml of the cell suspension.

•Determine the fluorescence intensity using a fluorescence microscope or a confocal laser microscope coupled with an image analyzer.

NOTE: Cell staining conditions differ by cell type, so it is necessary to optimize the conditions for each experiment.
Physical State :
Solid
Solubility :
Soluble in acetone, methanol, DMSO, and DMF. Insoluble in water.
Storage :
Store at -20° C
Boiling Point :
637.07° C at 760 mmHg (Predicted)
Density :
1.37 g/cm3 (Predicted)
Refractive Index :
n20D 1.69 (Predicted)
IC50 :
diacylglycerol kinase in isolated platelet membrane: IC50 = 300 nM; Inhibition of diacylglycerol kinase in intact platelets: IC50 = 120 nM; mitochondrial import inner membrane translocase subunit TIM23: IC50 = 5440 nM (Saccharomyces cerevisiae S288c)
Fluoresence :
λex 505 nm, λem 520 nm in DMSO
pK Values :
pKa: 11.24 (Predicted), pKb: 8.33 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
MDL Number :
MFCD00036969

Download SDS (MSDS)

Certificate of Analysis

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BCECF/AM (CAS 117464-70-7) Product Citations

See how others have used BCECF/AM (CAS 117464-70-7). Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 29731886  Kuang, Y.|Wang, S.|Tang, L.|Hai, J.|Yan, G.|Liao, L.| et al. 2018. Oncol Lett. 15: 7279-7290.

PMID: # 11548882  Miyashita, T. et al. 2001. Jpn. Circ. J. 65: 815-819.

Citations 1 to 2 of 2 total
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Rated 5 out of 5 by from Various publications cite BCECF/AM as a membrane Various publications cite BCECF/AM as a membrane-permeable fluorescent indicator for the measurement of cytoplasmic pH. -SCBT Publication Review
Date published: 2015-02-19
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