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Astra Blue (CAS 82864-57-1)

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Alternate Names:
Astra Blue is also known as Basic Blue 140.
Application:
Astra Blue is a 90% Dye content stain for oligo- and polysaccharides such as cellulose in plant tissues.
CAS Number:
82864-57-1
Molecular Weight:
1068.75
Molecular Formula:
C47H52CuN14O6S3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Astra Blue, known chemically as 1,3-Bis[4-(dimethylamino)phenyl]-1,3-propanedione bisbenzenesulfonate, is a cationic dye used primarily in botanical research for staining acidic plant cell components, such as lignin and cellulose. Its mechanism of action involves binding to the polysaccharide components within plant tissues, enabling detailed visualization of cell walls and other structural features under a microscope. Astra Blue is particularly valued for its ability to provide sharp contrast when paired with other dyes, such as safranin, in dual staining procedures where it offers a distinct color differentiation that enhances the microscopic examination of plant materials. This staining technique is crucial in the study of plant anatomy and physiology, helping researchers to observe and document the complex details of plant tissue organization and to assess changes in response to various environmental conditions or treatments. The dye′s strong affinity for the acidic components of cell walls allows for precise delineation of these structures, thereby facilitating a deeper understanding of plant structural biology and supporting studies in plant development and pathology. Astra Blue′s use in research is thus integral to advancing knowledge in plant sciences, providing essential insights into the cellular architecture and functional dynamics of plant tissues.


Astra Blue (CAS 82864-57-1) References

  1. A simple method that uses differential staining and light microscopy to assess the selectivity of wood delignification by white rot fungi.  |  Srebotnik, E. and Messner, K. 1994. Appl Environ Microbiol. 60: 1383-6. PMID: 16349245
  2. Combined histochemistry and autofluorescence for identifying lignin distribution in cell walls.  |  De Micco, V. and Aronne, G. 2007. Biotech Histochem. 82: 209-16. PMID: 18074267
  3. Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.  |  Korzhevskii, DE., et al. 2015. Eur J Histochem. 59: 2530. PMID: 26428887
  4. Impact of planetary ball mills on corn stover characteristics and enzymatic digestibility depending on grinding ball properties.  |  Lee, JH., et al. 2017. Bioresour Technol. 241: 1094-1100. PMID: 28642052
  5. Metachromatic staining and electron dense reaction of glycosaminoglycans by means of cuprolinic blue.  |  Juarranz, A., et al. 1987. Histochem J. 19: 1-6. PMID: 3583811
  6. Suitability of Mycelium-Reinforced Nanofiber Mats for Filtration of Different Dyes.  |  Heide, A., et al. 2023. Polymers (Basel). 15: PMID: 37836000
  7. Presence of mast cells in various oral mucosal sites in juvenile and adult rats.  |  Matsson, L. 1993. Scand J Dent Res. 101: 292-8. PMID: 7504320
  8. Astra blue and basic fuchsin double staining of plant materials.  |  Kraus, JE., et al. 1998. Biotech Histochem. 73: 235-43. PMID: 9829416
  9. Modified staining protocol with Safranin O and Astra Blue for the plant histology  |  Novikov, A. and Sup-Novikova, M. 2021. Plant introduction. 89/90: 110-113.
  10. Observing the Structure Diversity of Historic Heirloom Apple Tree (Malus domestica Borkh.) Wood in Central Slovakia  |  Slováčková B, Mišíková O. 2022. Diversity. 15(1): 15.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Astra Blue, 5 g

sc-214558A
5 g
$56.00

Astra Blue, 25 g

sc-214558
25 g
$156.00

Hi! Does Astra Blue work on pollen?

Asked by: IruNahir
Thank you for your question. Unfortunately we do not have any information regarding this biochemical working on pollen.
Answered by: Tech Service
Date published: 2019-04-02

What is the appropriate method for dissolution of this compound?

Asked by: two2igm05
Thank you for your question. Astra Blue, sc-214558, is soluble in water, methanol and DMSO.
Answered by: Chemical Support 4
Date published: 2023-09-13

How should I store this compound? Does it lose reactivity if it is stored in wrong conditions?

Asked by: two2igm05
Thank you for your question. We recommend to store this product, Astra Blue (CAS 82864-57-1), as desiccated at room temperature. The material is moisture sensitive, if stored incorrectly it could cause the material to lose reactivity.
Answered by: Technical Support
Date published: 2017-01-11
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Rated 5 out of 5 by from Astra blueFast delivery and stain was good , I used that for triple staining of starch .
Date published: 2025-07-24
Rated 5 out of 5 by from 染色很好进口试剂就是好,多次购买,发货快,服务态度好!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
Date published: 2021-11-23
Rated 5 out of 5 by from Various publications cite the use of AstraVarious publications cite the use of Astra blue as a stain for the detection of oligo- and polyssacharides in plant tissues. -SCBT Publication Review
Date published: 2015-02-04
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Astra Blue is rated 5.0 out of 5 by 3.
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