LOC728129 Activators
Chemical activators of LOC728129 can influence the protein's activity through diverse cellular pathways. Phorbol 12-myristate 13-acetate (PMA) and Bryostatin 1 are known to activate protein kinase C (PKC). This enzyme, upon activation, phosphorylates a variety of substrates, including LOC728129, resulting in its activation. Oleoyl-2-acetyl-sn-glycerol (OAG) serves a similar function as a diacylglycerol (DAG) analog, directly stimulating PKC and thus potentially enhancing the activity of LOC728129 through phosphorylation. Similarly, Forskolin increases the cellular concentration of cAMP, which activates protein kinase A (PKA). PKA then phosphorylates its specific protein targets, which may encompass LOC728129, thereby increasing its activity. The action of Epidermal Growth Factor (EGF) involves binding to its receptor EGFR, activating an intrinsic tyrosine kinase which initiates a cascade of phosphorylation events, and LOC728129 can be one of the proteins modified in this process.
Intracellular calcium levels are pivotal in regulating various proteins, and Ionomycin raises these levels, which can lead to the activation of calmodulin-dependent protein kinases (CaMKs). If LOC728129 is a substrate for CaMKs, its activity would be enhanced following Ionomycin exposure. Thapsigargin similarly causes an increase in cytosolic calcium by inhibiting the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), potentially leading to the activation of LOC728129 through CaMKs. Additionally, Okadaic Acid and Calyculin A, by inhibiting protein phosphatases, prevent the dephosphorylation of proteins. This sustained phosphorylation could maintain LOC728129 in an active state. Anisomycin, which activates stress-activated protein kinases, including JNK and p38 MAPK, could also result in the phosphorylation and subsequent activation of LOC728129 if it is within their substrate range. Lastly, Endothelin-1 stimulates its receptors to activate PKC, which may then phosphorylate LOC728129, while Ouabain, through inhibition of Na+/K+-ATPase, might indirectly activate signaling pathways that lead to the phosphorylation and activation of LOC728129.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which is known to phosphorylate and activate many proteins. PKC-mediated phosphorylation of LOC728129 would result in direct activation of the protein. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium, which can activate calmodulin-dependent protein kinases (CaMKs). These kinases can phosphorylate and activate certain proteins, and if LOC728129 is a substrate, its activity would be increased. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to increased cytosolic calcium levels, which in turn can activate CaMKs, potentially leading to the phosphorylation and activation of LOC728129. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid inhibits protein phosphatases, resulting in prolonged phosphorylation state of proteins, which can include LOC728129 if it is a phosphoprotein. This would lead to its sustained activation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is known to activate stress-activated protein kinase (SAPK) pathways, which could include JNK and p38 MAPK. These kinases can phosphorylate and activate specific proteins, and LOC728129 could be activated in this manner if it is part of the SAPK pathway. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $245.00 | 9 | |
Bryostatin 1 binds to and modulates PKC activity, leading to its activation. Activated PKC can phosphorylate and activate numerous proteins, including LOC728129 if it is a PKC substrate. | ||||||
1-Oleoyl-2-acetyl-sn-glycerol (OAG) | 86390-77-4 | sc-200417 sc-200417A | 10 mg 50 mg | $119.00 $453.00 | 1 | |
OAG serves as a DAG analog that activates PKC, leading to phosphorylation and activation of protein substrates, potentially including LOC728129 if it is a target of PKC. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A, like Okadaic Acid, inhibits protein phosphatases, particularly PP1 and PP2A, leading to increased phosphorylation and activation of proteins. LOC728129 activation could occur if it is normally regulated by dephosphorylation. | ||||||
Ouabain-d3 (Major) | sc-478417 | 1 mg | $516.00 | |||
Ouabain specifically inhibits Na+/K+-ATPase, which can lead to changes in cellular ion gradients and subsequent activation of signal transduction pathways, including those involving phosphorylation of proteins. If LOC728129 is activated by such signaling cascades, Ouabain would be an indirect activator. | ||||||