KBTBD13 Activators
Chemical activators of kelch repeat and BTB domain containing 13 can initiate a range of biochemical pathways leading to its functional activation. Phorbol 12-myristate 13-acetate activates protein kinase C (PKC), a family of enzymes that play a key role in the regulation of kelch repeat and BTB domain containing 13 through phosphorylation, leading to cellular responses. Forskolin, by increasing intracellular cAMP levels, triggers the activation of protein kinase A (PKA), which in turn can phosphorylate kelch repeat and BTB domain containing 13, altering its activity. Similarly, dibutyryl-cAMP, a cAMP analog, activates PKA, thereby promoting the phosphorylation and activation of kelch repeat and BTB domain containing 13. Ionomycin, by increasing intracellular calcium levels, can activate calcium/calmodulin-dependent protein kinases, which may target kelch repeat and BTB domain containing 13 for phosphorylation and subsequent activation.
In addition to these activators, Epidermal Growth Factor (EGF) engages its receptor tyrosine kinase, initiating a cascade that can lead to the phosphorylation of kelch repeat and BTB domain containing 13. Zinc sulfate serves as a second messenger that can activate kinases which phosphorylate kelch repeat and BTB domain containing 13. Hydrogen peroxide, through oxidative mechanisms, can modify signaling pathways and thus, the redox state of kelch repeat and BTB domain containing 13, influencing its function. Thapsigargin disrupts calcium homeostasis and can also lead to the activation of kelch repeat and BTB domain containing 13 via calcium-dependent phosphorylation mechanisms. Inhibitors like calyculin A and okadaic acid, which inhibit protein phosphatases, prevent the dephosphorylation of proteins including kelch repeat and BTB domain containing 13, thus maintaining it in an activated state. Lastly, Bisindolylmaleimide I, although primarily a PKC inhibitor, at low concentrations can paradoxically activate PKC, and hence, can indirectly promote the activation of kelch repeat and BTB domain containing 13 through phosphorylation events.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
Phorbol 12-myristate 13-acetate activates protein kinase C (PKC), which can phosphorylate kelch repeat and BTB domain containing 13, leading to its functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin increases intracellular calcium concentrations, which can activate calcium/calmodulin-dependent protein kinases (CaMKs). These kinases can then phosphorylate and activate kelch repeat and BTB domain containing 13 by promoting its binding to specific substrates or partners within its pathway. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc sulfate can act as a second messenger within cells and may activate kinases that phosphorylate kelch repeat and BTB domain containing 13, thereby enhancing its functional activity in the cell. | ||||||
Hydrogen Peroxide | 7722-84-1 | sc-203336 sc-203336A sc-203336B | 100 ml 500 ml 3.8 L | $31.00 $61.00 $95.00 | 28 | |
Hydrogen peroxide can act as an oxidative agent that modulates signaling pathways by oxidative modification of proteins, which may include the activation of kelch repeat and BTB domain containing 13 through the alteration of its redox state, affecting its interaction with binding partners. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin disrupts calcium homeostasis by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), leading to increased cytosolic calcium levels, which can activate kelch repeat and BTB domain containing 13 through calcium-dependent phosphorylation mechanisms. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Calyculin A inhibits protein phosphatases, which leads to an increase in the phosphorylation state of cellular proteins. This can result in the activation of kelch repeat and BTB domain containing 13 by preventing its dephosphorylation, thus maintaining the protein in an active state. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases 1 and 2A, leading to increased phosphorylation within the cell. This can cause the activation of kelch repeat and BTB domain containing 13 by maintaining its phosphorylation levels, which is necessary for its activity. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP, a cell-permeable cAMP analog, activates cAMP-dependent PKA. PKA can then phosphorylate kelch repeat and BTB domain containing 13, resulting in its activation and facilitation of its role in cellular processes. | ||||||
Bisindolylmaleimide I (GF 109203X) | 133052-90-1 | sc-24003A sc-24003 | 1 mg 5 mg | $105.00 $242.00 | 36 | |
Bisindolylmaleimide I is a potent and selective inhibitor of PKC, but it can also function paradoxically in certain contexts to activate PKCs at low concentrations. This can lead to the activation of kelch repeat and BTB domain containing 13 indirectly by enhancing PKC-mediated phosphorylation events that activate the protein. | ||||||
8-Bromoadenosine 3′,5′-cyclic monophosphate | 23583-48-4 | sc-217493B sc-217493 sc-217493A sc-217493C sc-217493D | 25 mg 50 mg 100 mg 250 mg 500 mg | $108.00 $169.00 $295.00 $561.00 $835.00 | 2 | |
8-Bromoadenosine 3',5'-cyclic monophosphate is a cell-permeable cAMP analog that activates PKA. The activation of PKA can lead to the phosphorylation and subsequent activation of kelch repeat and BTB domain containing 13, enhancing its functional role in the cell. | ||||||