GEN1 Activators
GEN1 activators, as described in the context of the given list, are chemicals that can indirectly promote the activity or necessity of GEN1 within the cell. GEN1 functions as a Holliday junction resolvase, an essential component in the resolution of Holliday junctions that arise during processes like homologous recombination and DNA repair.
A significant portion of these activators disrupts DNA integrity or impedes the regular progression of DNA replication. For instance, Hydroxyurea hinders ribonucleotide reductase, causing replication forks to stall and eventually collapse into DNA breaks. These breaks then necessitate repair through recombination, wherein GEN1's Holliday junction resolving function can be crucial. Similarly, chemicals such as Cisplatin introduce DNA crosslinks, and Olaparib, a PARP inhibitor, escalates single-strand breaks into double-strand breaks. Both scenarios emphasize the need for homologous recombination, drawing attention to GEN1's role. Topoisomerase stabilizers like Camptothecin and Etoposide generate DNA breaks, again underlining the significance of GEN1 in DNA repair pathways. Moreover, DNA damaging agents, including Zeocin, and Mitomycin C, create disruptions in DNA that activate repair mechanisms where GEN1 can be instrumental.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Hydroxyurea | 127-07-1 | sc-29061 sc-29061A | 5 g 25 g | $78.00 $260.00 | 18 | |
An inhibitor of ribonucleotide reductase, which leads to stalled replication forks. These forks can then collapse into DNA breaks requiring recombination repair. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $138.00 $380.00 | 101 | |
Introduces DNA crosslinks which need to be repaired, often via homologous recombination processes where GEN1's function may be highlighted. | ||||||
Olaparib | 763113-22-0 | sc-302017 sc-302017A sc-302017B | 250 mg 500 mg 1 g | $210.00 $305.00 $495.00 | 10 | |
PARP inhibitor that causes an accumulation of single-strand breaks, which can then escalate to double-strand breaks, thus implicating homologous recombination. | ||||||
Camptothecin | 7689-03-4 | sc-200871 sc-200871A sc-200871B | 50 mg 250 mg 100 mg | $58.00 $186.00 $94.00 | 21 | |
Stabilizes DNA topoisomerase I covalent complexes, leading to DNA breaks that require resolution through homologous recombination. | ||||||
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $84.00 $306.00 $1104.00 | 30 | |
Inhibits DNA polymerase, causing replication stress and potentially increasing the need for GEN1 activity during Holliday junction resolution. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
Stabilizes DNA topoisomerase II covalent complexes, leading to DNA breaks that may require GEN1 activity. | ||||||
Mitomycin C | 50-07-7 | sc-3514A sc-3514 sc-3514B | 2 mg 5 mg 10 mg | $66.00 $101.00 $143.00 | 85 | |
Crosslinks DNA which can then necessitate homologous recombination and GEN1 function for resolution. | ||||||
Bleomycin | 11056-06-7 | sc-507293 | 5 mg | $275.00 | 5 | |
Induces DNA breaks, promoting the utilization of the homologous recombination pathway and the potential need for GEN1's Holliday junction resolving activity. | ||||||
Methyl methanesulfonate | 66-27-3 | sc-250376 sc-250376A | 5 g 25 g | $56.00 $133.00 | 2 | |
Alkylating agent that introduces lesions repaired via homologous recombination. | ||||||
Chlorambucil | 305-03-3 | sc-204682 sc-204682A | 250 mg 1 g | $52.00 $122.00 | 3 | |
Alkylating agent that can introduce DNA crosslinks and lesions repaired through recombination. | ||||||