GATC Inhibitors
Chemical inhibitors of GATC can impede its function through various mechanisms that involve interference with DNA synthesis and methylation processes. Methotrexate directly targets the thymidine synthesis pathway by competitively inhibiting dihydrofolate reductase (DHFR), which is vital for DNA synthesis, thereby limiting the availability of thymidine triphosphate (TTP), a necessary substrate for DNA replication and subsequently for GATC's methylation activity. Similarly, 5-Azacytidine and Decitabine act as nucleoside analogs that get incorporated into DNA and RNA. These compounds inhibit DNA methyltransferases, thus reducing the methylation of DNA which is crucial for GATC function. By altering the normal methylation patterns, GATC's ability to interact with DNA and exert its methylation effects is inhibited.
Zebularine and RG108, both DNA methyltransferase inhibitors, prevent the methylation of DNA, creating an abnormal methylation landscape wherein GATC cannot perform its normal role in DNA methylation. SGI-1027 also inhibits DNA methyltransferases, which diminishes the methylation on which GATC depends for its activity. Procaine and Hydralazine, though not specific to any one DNA methyltransferase, reduce overall DNA methylation, which would lead to a decrease in the functional methylation activity of GATC. Epigallocatechin gallate further impedes DNA methyltransferases, resulting in the inhibition of GATC by decreasing the methylated DNA substrates necessary for its action. Disulfiram's capability to chelate copper, a cofactor essential for many enzymes, can interfere with the catalytic activity of GATC that requires metal ions. Mithramycin A binds preferentially to GC-rich sequences in DNA, which prevents proteins, including GATC, from accessing their DNA targets, thus inhibiting their activity. Finally, Cytarabine, a nucleoside analog of cytidine, incorporates into DNA and disrupts DNA polymerases. This disruption of DNA replication can inhibit GATC by preventing the normal progression of replication forks where GATC would typically be active, thereby hindering its ability to participate in DNA methylation.
Items 1 to 10 of 12 total
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Methotrexate | 59-05-2 | sc-3507 sc-3507A | 100 mg 500 mg | $94.00 $213.00 | 33 | |
Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme critical in the thymidine synthesis pathway. Since GATC relies on DNA synthesis for its functional role in DNA methylation, inhibiting thymidine synthesis with Methotrexate will prevent GATC from performing its methylation activity due to lack of substrate. | ||||||
5-Azacytidine | 320-67-2 | sc-221003 | 500 mg | $280.00 | 4 | |
5-Azacytidine incorporates into RNA and DNA and inhibits DNA methyltransferase, which would interfere with GATC activity by reducing methylation substrates and altering the methylation landscape that GATC interacts with during DNA methylation processes. | ||||||
5-Aza-2′-Deoxycytidine | 2353-33-5 | sc-202424 sc-202424A sc-202424B | 25 mg 100 mg 250 mg | $218.00 $322.00 $426.00 | 7 | |
Decitabine is a cytidine analog that inhibits DNA methyltransferases, which would inhibit GATC function by hypomethylating DNA sites that are normally substrates for GATC, altering its methylation-dependent activities. | ||||||
Zebularine | 3690-10-6 | sc-203315 sc-203315A sc-203315B | 10 mg 25 mg 100 mg | $129.00 $284.00 $1004.00 | 3 | |
Zebularine acts as a DNA methyltransferase inhibitor. By preventing methylation, it would create an abnormal methylation pattern, which would inhibit the methylation function of GATC by preventing its normal interaction with DNA. | ||||||
RG 108 | 48208-26-0 | sc-204235 sc-204235A | 10 mg 50 mg | $131.00 $515.00 | 2 | |
RG108 is a non-nucleoside DNA methyltransferase inhibitor. It would block the methylation of DNA by GATC by directly inhibiting the enzymes that methylate the DNA substrates GATC normally acts upon. | ||||||
SGI-1027 | 1020149-73-8 | sc-473875 | 10 mg | $213.00 | ||
SGI-1027 is a small molecule inhibitor of DNA methyltransferases. It would inhibit GATC by reducing the methylation of DNA, thus altering GATC's ability to interact with normally methylated regions of DNA. | ||||||
Procaine | 59-46-1 | sc-296134 sc-296134A sc-296134B sc-296134C | 25 g 50 g 500 g 1 kg | $110.00 $193.00 $407.00 $628.00 | 1 | |
Procaine is a DNA methylation inhibitor that can non-specifically inhibit DNA methyltransferases, thereby potentially reducing the methylation activity of GATC through substrate limitation. | ||||||
Hydralazine-15N4 Hydrochloride | 304-20-1 (unlabeled) | sc-490605 | 1 mg | $480.00 | ||
Hydralazine is known to inhibit DNA methyltransferase and could limit the methylation capacity of GATC by altering the normal methylation patterns of DNA. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
Epigallocatechin gallate has been found to inhibit DNA methyltransferases, which would in turn inhibit the functional activity of GATC by reducing the availability of methylated DNA substrates. | ||||||
Disulfiram | 97-77-8 | sc-205654 sc-205654A | 50 g 100 g | $53.00 $89.00 | 7 | |
Disulfiram can chelate copper, an essential cofactor for various enzymes, and thereby could inhibit the activity of GATC by disrupting enzyme function that requires metal cofactors. | ||||||