FBXO46 Activators
FBXO46 employ various mechanisms to enhance its function by modulating the phosphorylation status of the protein. Forskolin is a direct stimulator of adenylate cyclase, which increases the levels of cAMP within the cell, leading to the activation of protein kinase A (PKA). PKA then can phosphorylate FBXO46, potentially increasing its ubiquitination activity. Similarly, IBMX, by inhibiting phosphodiesterases, sustains elevated levels of cAMP and thus PKA activity, indirectly promoting FBXO46 phosphorylation. PGE2 operates through G-protein-coupled receptors to also raise cAMP levels and activate PKA, which, in turn, can phosphorylate FBXO46. Epinephrine and isoproterenol, both adrenergic agonists, trigger similar cAMP-mediated pathways resulting in PKA activation, which then can target FBXO46 for phosphorylation.
cAMP-mediated pathways, other activators work through different mechanisms. Anisomycin, which inhibits protein synthesis, can activate stress-activated protein kinases like JNK, which can then directly phosphorylate FBXO46. Okadaic acid and Calyculin A, as inhibitors of protein phosphatases PP1 and PP2A, prevent the dephosphorylation of proteins, possibly resulting in enhanced or prolonged phosphorylation of FBXO46. Dibutyryl-cAMP, a cAMP analog, bypasses cell surface receptors to directly activate PKA and thus can lead to FBXO46 phosphorylation. Rolipram and Zaprinast specifically inhibit PDE4 and PDE5, respectively, leading to an accumulation of cAMP and subsequent activation of PKA, which then can phosphorylate FBXO46. Lastly, Sp-8-Br-cAMPS, a stable cAMP analog, activates PKA, which is capable of phosphorylating FBXO46, enhancing its activity within the cell's ubiquitination pathways.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
Isobutylmethylxanthine (IBMX) is a non-selective inhibitor of phosphodiesterases, enzymes that degrade cAMP. By preventing cAMP breakdown, IBMX indirectly sustains PKA activation which in turn can enhance FBXO46 function through phosphorylation-dependent mechanisms. | ||||||
PGE2 | 363-24-6 | sc-201225 sc-201225C sc-201225A sc-201225B | 1 mg 5 mg 10 mg 50 mg | $57.00 $159.00 $275.00 $678.00 | 37 | |
Prostaglandin E2 (PGE2) binds to its G-protein-coupled receptors, leading to an increase in intracellular cAMP levels, thereby activating PKA. Activated PKA can phosphorylate various substrates, potentially including FBXO46, thus enhancing its activity in the cell. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a protein synthesis inhibitor, which can activate stress-activated protein kinases like JNK. JNK activation can lead to the phosphorylation of several proteins, potentially including FBXO46, which might result in its enhanced functional activity. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic acid is a potent inhibitor of protein phosphatases PP1 and PP2A, leading to increased phosphorylation levels of cellular proteins due to reduced dephosphorylation. This potentially includes FBXO46, resulting in its increased activity due to sustained phosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to okadaic acid, Calyculin A is an inhibitor of protein phosphatases PP1 and PP2A, which could lead to an accumulation of phosphorylated FBXO46, thus maintaining or enhancing its activity. | ||||||
(−)-Epinephrine | 51-43-4 | sc-205674 sc-205674A sc-205674B sc-205674C sc-205674D | 1 g 5 g 10 g 100 g 1 kg | $41.00 $104.00 $201.00 $1774.00 $16500.00 | ||
Epinephrine binds to adrenergic receptors, which can lead to the activation of adenylate cyclase and an increase in cAMP levels. This elevation in cAMP can activate PKA, which may phosphorylate and thus enhance the activity of FBXO46. | ||||||
Isoproterenol Hydrochloride | 51-30-9 | sc-202188 sc-202188A | 100 mg 500 mg | $28.00 $38.00 | 5 | |
Isoproterenol is a synthetic catecholamine that activates beta-adrenergic receptors, leading to increased cAMP production and PKA activation. PKA can phosphorylate many proteins, which could include FBXO46, potentially leading to its functional activation. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable analog of cAMP that can activate PKA directly within the cell, bypassing the receptor-mediated pathway. This direct activation of PKA can lead to phosphorylation and activation of FBXO46 in the ubiquitination pathway. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $77.00 $216.00 | 18 | |
Rolipram selectively inhibits phosphodiesterase 4 (PDE4), leading to increased cAMP levels and subsequent activation of PKA. This activation can promote phosphorylation and subsequent activation of substrates, including potentially FBXO46, involved in protein ubiquitination. | ||||||
Zaprinast (M&B 22948) | 37762-06-4 | sc-201206 sc-201206A | 25 mg 100 mg | $105.00 $250.00 | 8 | |
Zaprinast is primarily known as a selective inhibitor of phosphodiesterase 5 (PDE5), but it also has effects on PDE9, leading to increased cAMP levels in certain cell types. The resulting PKA activation could enhance the phosphorylation and activity of FBXO46. | ||||||