DNA2L Activators
Chemicals classified as DNA2L Activators influence the protein's activity mainly by affecting DNA replication and repair pathways. DNA2L is a versatile protein functioning as a helicase and an endonuclease, known for processing single-stranded DNA (ssDNA) during DNA replication and repair. The first group of chemicals, including Aphidicolin, directly stall DNA replication forks. The stalling leads to the accumulation of ssDNA, which requires processing. DNA2L is then recruited to the stalled replication forks where it uses its helicase and endonucuclease activities to process the accumulated ssDNA. In essence, these chemicals create a situation that necessitates the action of DNA2L, indirectly enhancing its functional activity.
The second group of chemicals, including Etoposide, and Bleomycin, act by inducing DNA damage. This damage, either in the form of DNA double-strand breaks or DNA single-strand breaks, can lead to the generation of ssDNA during the DNA repair process. DNA2L, with its ssDNA processing abilities, is then called upon to act on this ssDNA, leading to an enhancement of its functional activity. In a similar vein, DNA crosslinking agents like Cisplatin and Mitomycin C, or DNA alkylating agents like Chlorambucil, also cause DNA damage that necessitates the involvement of DNA2L. The third group of chemicals, including Gemcitabine, are nucleoside analogs that get incorporated into DNA during DNA replication. The presence of these analogs in the DNA can stall replication forks, leading to the generation of ssDNA. Once again, this ssDNA needs to be processed, and DNA2L is recruited for this task, enhancing its functional activity. Finally, Doxorubicin, a DNA intercalating agent, causes DNA damage, leading to stalled replication forks and increased ssDNA. This ssDNA is a substrate for DNA2L, and its presence indirectly enhances the functional activity of DNA2L. Overall, all these chemicals act by creating a situation where the ssDNA processing activities of DNA2L are required, leading to an enhancement of its functional activity.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Aphidicolin | 38966-21-1 | sc-201535 sc-201535A sc-201535B | 1 mg 5 mg 25 mg | $84.00 $306.00 $1104.00 | 30 | |
Aphidicolin inhibits DNA polymerases, causing stalling of replication forks. This results in increased ssDNA, which can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Etoposide (VP-16) | 33419-42-0 | sc-3512B sc-3512 sc-3512A | 10 mg 100 mg 500 mg | $51.00 $231.00 $523.00 | 63 | |
Etoposide inhibits topoisomerase II, leading to DNA double-strand breaks. During repair, ssDNA is generated, which can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Bleomycin Sulfate | 9041-93-4 | sc-200134 sc-200134A sc-200134B sc-200134C | 10 mg 50 mg 100 mg 500 mg | $210.00 $624.00 $1040.00 $2913.00 | 38 | |
Bleomycin causes DNA strand breaks, which leads to the generation of ssDNA. This ssDNA can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Olaparib | 763113-22-0 | sc-302017 sc-302017A sc-302017B | 250 mg 500 mg 1 g | $210.00 $305.00 $495.00 | 10 | |
Olaparib inhibits PARP, an enzyme involved in DNA repair. This leads to increased DNA damage and ssDNA, which can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Cisplatin | 15663-27-1 | sc-200896 sc-200896A | 100 mg 500 mg | $138.00 $380.00 | 101 | |
Cisplatin causes DNA crosslinking, leading to stalling of replication forks and increased ssDNA. This ssDNA can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Mitomycin C | 50-07-7 | sc-3514A sc-3514 sc-3514B | 2 mg 5 mg 10 mg | $66.00 $101.00 $143.00 | 85 | |
Mitomycin C crosslinks DNA, causing replication fork stalling and increased ssDNA, which can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Chlorambucil | 305-03-3 | sc-204682 sc-204682A | 250 mg 1 g | $52.00 $122.00 | 3 | |
Chlorambucil alkylates and crosslinks DNA, leading to replication fork stalling and increased ssDNA. This ssDNA can be processed by DNA2L, thus enhancing its functional activity. | ||||||
2′-Deoxy-2′,2′-difluorocytidine | 95058-81-4 | sc-275523 sc-275523A | 1 g 5 g | $56.00 $128.00 | ||
2'-Deoxy-2',2'-difluorocytidine (Gemcitabine) is incorporated into DNA, causing stalling of replication forks and increased ssDNA. This ssDNA can be processed by DNA2L, thus enhancing its functional activity. | ||||||
Doxorubicin | 23214-92-8 | sc-280681 sc-280681A | 1 mg 5 mg | $176.00 $426.00 | 43 | |
Doxorubicin intercalates into DNA, causing DNA damage, replication fork stalling, and increased ssDNA. This ssDNA can be processed by DNA2L, thus enhancing its functional activity. | ||||||