DENND2A Activators
DENND2A Activators comprise a diverse array of chemical compounds that indirectly stimulate the functional activity of DENND2A, which acts as a guanine nucleotide exchange factor for the small GTPase Rab35, involved in endocytic recycling. Forskolin and 8-Bromo-cAMP, through their ability to increase cellular cAMP levels, enhance DENND2A's activity by fostering the activation of Rab35, thereby facilitating its role in membrane trafficking. Similarly, IBMX and Rolipram contribute to the activation of DENND2A by inhibiting phosphodiesterases, resulting in elevated cAMP levels and subsequent Rab35 activation. Ionomycin, by raising intracellular calcium levels, and PMA, as a PKC activator, can modify the cellular environment to favor DENND2A's interaction with Rab35 through calmodulin-dependent pathways and phosphorylation of associated proteins, respectively. Epigallocatechin gallate (EGCG) supports DENND2A activity by inhibiting kinases that might otherwise suppress Rab35 activation, shifting the signaling equilibrium towards DENND2A's functional pathway.
Furthermore, the PI3K inhibitor LY294002 indirectly enhances DENND2A by modulating downstream signals that can impact Rab35 activation, while U0126 alters MAPK pathway signaling, potentially favoring DENND2A-mediated processes. NSC 23766 and ML141, by targeting small GTPases Rac1 and Cdc42, may promote DENND2A activity by modulating the actin cytoskeleton, thus indirectly influencing Rab35's role in trafficking. Gö6983, despite being a broad-spectrum PKC inhibitor, may paradoxically augment DENND2A's function by selectively downregulating PKC isoforms that negatively regulate Rab35-associated pathways. Collectively, these activators do not enhance DENND2A by direct stimulation but rather by influencing a network of cellular processes and pathways that are conducive to DENND2A's role in regulating Rab35 and endocytic trafficking.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Forskolin | 66575-29-9 | sc-3562 sc-3562A sc-3562B sc-3562C sc-3562D | 5 mg 50 mg 1 g 2 g 5 g | $78.00 $153.00 $740.00 $1413.00 $2091.00 | 73 | |
Forskolin is an activator of adenylate cyclase, leading to increased cAMP levels in cells. The rise in cAMP can enhance DENND2A activity by promoting its interaction with Rab35, a small GTPase involved in endocytic recycling, which DENND2A functions as a guanine nucleotide exchange factor for. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
This cAMP analog is resistant to degradation and serves as a direct activator of cAMP-dependent pathways, potentially enhancing DENND2A's activity by augmenting Rab35 activation through increased cAMP levels. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC) which could enhance DENND2A activity indirectly by phosphorylation of proteins involved in the same trafficking pathways as DENND2A, thereby influencing membrane dynamics and Rab35 activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Elevated calcium can promote DENND2A activity by stimulating calmodulin-dependent pathways, potentially affecting Rab35 interaction and membrane trafficking. | ||||||
(−)-Epigallocatechin Gallate | 989-51-5 | sc-200802 sc-200802A sc-200802B sc-200802C sc-200802D sc-200802E | 10 mg 50 mg 100 mg 500 mg 1 g 10 g | $43.00 $73.00 $126.00 $243.00 $530.00 $1259.00 | 11 | |
EGCG is known to inhibit several protein kinases; it could enhance DENND2A activity by inhibiting competing kinases, thereby shifting the balance towards pathways that activate Rab35 and its associated endocytic recycling processes. | ||||||
IBMX | 28822-58-4 | sc-201188 sc-201188B sc-201188A | 200 mg 500 mg 1 g | $260.00 $350.00 $500.00 | 34 | |
IBMX is a non-selective inhibitor of phosphodiesterases, leading to increased cAMP levels. This elevation can indirectly enhance DENND2A activity by promoting Rab35 activation and facilitating endocytic recycling. | ||||||
Rolipram | 61413-54-5 | sc-3563 sc-3563A | 5 mg 50 mg | $77.00 $216.00 | 18 | |
Rolipram is a selective inhibitor of phosphodiesterase 4, which specifically increases cAMP in certain cell types. This increase could enhance DENND2A activity by stimulating Rab35-dependent pathways. | ||||||
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a PI3K inhibitor, which could indirectly enhance DENND2A activity by modulating downstream signaling that affects Rab35 activation, as PI3K signaling is involved in many aspects of intracellular trafficking. | ||||||
U-0126 | 109511-58-2 | sc-222395 sc-222395A | 1 mg 5 mg | $64.00 $246.00 | 136 | |
U0126 is an inhibitor of MEK1/2, and by modulating the MAPK pathway, it could shift the cellular signaling balance in favor of pathways that stimulate DENND2A activity through its effect on Rab35. | ||||||
NSC 23766 | 733767-34-5 | sc-204823 sc-204823A | 10 mg 50 mg | $151.00 $609.00 | 75 | |
NSC 23766 inhibits Rac1, which may indirectly enhance DENND2A activity by altering actin cytoskeleton dynamics and thereby potentially affecting membrane trafficking processes involving Rab35. | ||||||