DDX38 Activators
DDX38 Activators encompass a range of chemical compounds that indirectly amplify the helicase activity of DDX38. Central to this group is ATP, which is essential for the catalytic action of DDX38, as it binds and hydrolyzes to provide the necessary energy for unwinding RNA. Magnesium chloride complements the action of ATP, ensuring efficient ATPase activity by acting as an essential cofactor. The functional specificity of DDX38 is targeted by Poly(U) RNA, which acts as a substrate, enabling DDX38 to perform its helicase function. The ionic environment, optimized by KCl, is crucial for the electrostatic interactions between DDX38 and its substrates, which is fundamental for helicase activity. ZnSO4 may contribute to the structural stability of DDX38, potentially leading to an enhanced functional state, while the disodium salt of ATP offers an alternative form of energy that could be readily utilized by DDX38.
The activity of DDX38 is further influenced by compounds that stabilize the protein and its interactions. Glycerol functions as a stabilizing agent, maintaining DDX38's conformation and, consequently, its activity during catalysis. Antioxidant properties of 3,4-Dihydroxybenzoic acid may preserve the functional integrity of DDX38, preventing oxidative damage that could impair helicase activity. Spermidine has a role in stabilizing RNA structures, which could enhance the RNA helicase activity of DDX38 by maintaining the integrity of its RNA substrates. The reducing agent DTT ensures that DDX38 maintains its proper folding and active site configuration by keeping cysteine residues in a reduced state. Sodium molybdate could provide indirect support to DDX38's structure, while β-Nicotinamide mononucleotide, by boosting cellular NAD+ levels, supports the overall energy status of the cell, indirectly favoring DDX38's ATP-dependent activities. Collectively, these activators orchestrate a conducive biochemical environment that maximizes the helicase function of DDX38 without altering its expression levels.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
ADP | 58-64-0 | sc-507362 | 5 g | $53.00 | ||
As a helicase, DDX38 requires ATP for its activity. ATP binding results in the activation of its helicase function, unwinding RNA or DNA duplexes during various cellular processes. | ||||||
Magnesium chloride | 7786-30-3 | sc-255260C sc-255260B sc-255260 sc-255260A | 10 g 25 g 100 g 500 g | $27.00 $34.00 $47.00 $123.00 | 2 | |
Magnesium ions are essential co-factors for ATPase activity. Mg2+ presence is critical for the proper function of DDX38 as it facilitates the hydrolysis of ATP, which is necessary for the helicase action of DDX38. | ||||||
Potassium Chloride | 7447-40-7 | sc-203207 sc-203207A sc-203207B sc-203207C | 500 g 2 kg 5 kg 10 kg | $25.00 $56.00 $104.00 $183.00 | 5 | |
Potassium chloride affects ionic strength and can influence the RNA helicase activity of DDX38 by altering the electrostatic interactions between the protein and its nucleic acid substrates. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $47.00 | ||
Zinc sulfate can act as a stabilizing agent for RNA helicases like DDX38. Zinc ions may enhance the structural stability of DDX38, thereby potentially improving its functional activity. | ||||||
Adenosine 5′-Triphosphate, disodium salt | 987-65-5 | sc-202040 sc-202040A | 1 g 5 g | $38.00 $74.00 | 9 | |
Similar to ATP, this salt form can serve as an energy source for DDX38, enhancing its helicase function through the provision of phosphate groups necessary for the unwinding mechanism. | ||||||
Glycerol | 56-81-5 | sc-29095A sc-29095 | 100 ml 1 L | $55.00 $150.00 | 12 | |
Glycerol is commonly used as a stabilizing agent in protein assays. It can enhance the functional activity of DDX38 by maintaining its conformation and stability during biochemical reactions. | ||||||
Protocatechuic acid | 99-50-3 | sc-205818 sc-205818A | 25 g 50 g | $126.00 $255.00 | 9 | |
This compound can act as an antioxidant, possibly maintaining the integrity of DDX38 protein structure by preventing oxidative damage and thus preserving its activity. | ||||||
Spermidine | 124-20-9 | sc-215900 sc-215900B sc-215900A | 1 g 25 g 5 g | $56.00 $595.00 $173.00 | ||
Spermidine stabilizes ribonucleoprotein structures and could enhance the RNA helicase activity of DDX38 by maintaining the integrity of RNA substrates. | ||||||
Sodium molybdate | 7631-95-0 | sc-236912 sc-236912A sc-236912B | 5 g 100 g 500 g | $55.00 $82.00 $316.00 | 1 | |
Sodium molybdate may act as a cofactor for certain enzymes and could indirectly enhance the activity of DDX38 by stabilizing its tertiary or quaternary structure. | ||||||
β-Nicotinamide mononucleotide | 1094-61-7 | sc-212376 sc-212376A sc-212376B sc-212376C sc-212376D | 25 mg 100 mg 1 g 2 g 5 g | $92.00 $269.00 $337.00 $510.00 $969.00 | 4 | |
As a precursor for NAD+, β-Nicotinamide mononucleotide could support the energy status of the cell, indirectly enhancing the ATP-dependent helicase activity of DDX38. | ||||||