DDX38 Antibody (A-8): sc-137218
- DDX38 Antibody (A-8) is a mouse monoclonal IgG1 κ DDX38 antibody provided at 200 µg/ml
- raised against amino acids 282-510 mapping within an internal region of DDX38 of human origin
- DDX38 Antibody (A-8) is recommended for detection of DDX38 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-DDX38 Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX38 Antibody (A-8) for WB applications. These reagents are now offered in bundles with DDX38 Antibody (A-8) (see ordering information below).
DDX38 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDX38 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDX38 Antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX38 protein, also known as pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, consists of 1,227 amino acids and is encoded by human gene DDX38. DDX38 is a member of the DEAD-box helicase family, specifically the DEAH subfamily, and features one helicase ATP-binding domain and one helicase C-terminal domain, which are crucial for function as an ATP-dependent RNA helicase. DDX38 plays a key role in RNA metabolism, participating in essential processes such as transcription, splicing, and translation, where DDX38 modulates RNA structure by utilizing energy from nucleoside triphosphate hydrolysis. Understanding DDX38 function is vital, as DDX38 significantly influences gene expression regulation and proper processing of pre-mRNA, which is critical for maintaining cellular function and responding to various physiological stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DDX38 Antibody (A-8) References:
- Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. | Das, R., et al. 2000. Mol Cell. 5: 779-87. PMID: 10882114
- The transcriptional landscape of the mammalian genome. | Carninci, P., et al. 2005. Science. 309: 1559-63. PMID: 16141072
- Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. | Ancelin, K., et al. 2006. Nat Cell Biol. 8: 623-30. PMID: 16699504
- Splicing fidelity revisited. | Query, CC. and Konarska, MM. 2006. Nat Struct Mol Biol. 13: 472-4. PMID: 16757943
- U2 snRNP binds intronless histone pre-mRNAs to facilitate U7-snRNP-dependent 3' end formation. | Friend, K., et al. 2007. Mol Cell. 28: 240-52. PMID: 17964263
- Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing. | Zhou, Z. and Reed, R. 1998. EMBO J. 17: 2095-106. PMID: 9524131
- The mammalian homologue of Prp16p is overexpressed in a cell line tolerant to Leflunomide, a new immunoregulatory drug effective against rheumatoid arthritis. | Ortlepp, D., et al. 1998. RNA. 4: 1007-18. PMID: 9701291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX38 Antibody (A-8) | sc-137218 | 200 µg/ml | $322.00 | |||
DDX38 Antibody (A-8): m-IgGκ BP-HRP Bundle | sc-521454 | 200 µg Ab, 40 µg BP | $361.00 | |||
DDX38 Antibody (A-8): m-IgG1 BP-HRP Bundle | sc-533492 | 200 µg Ab; 20 µg BP | $361.00 | |||
DDX38 Antibody (A-8) AC | sc-137218 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DDX38 Antibody (A-8) HRP | sc-137218 HRP | 200 µg/ml | $322.00 | |||
DDX38 Antibody (A-8) FITC | sc-137218 FITC | 200 µg/ml | $336.00 | |||
DDX38 Antibody (A-8) PE | sc-137218 PE | 200 µg/ml | $349.00 | |||
DDX38 Antibody (A-8) Alexa Fluor® 488 | sc-137218 AF488 | 200 µg/ml | $364.00 | |||
DDX38 Antibody (A-8) Alexa Fluor® 546 | sc-137218 AF546 | 200 µg/ml | $364.00 | |||
DDX38 Antibody (A-8) Alexa Fluor® 594 | sc-137218 AF594 | 200 µg/ml | $364.00 | |||
DDX38 Antibody (A-8) Alexa Fluor® 647 | sc-137218 AF647 | 200 µg/ml | $364.00 | |||
DDX38 Antibody (A-8) Alexa Fluor® 680 | sc-137218 AF680 | 200 µg/ml | $364.00 | |||
DDX38 Antibody (A-8) Alexa Fluor® 790 | sc-137218 AF790 | 200 µg/ml | $364.00 |