Dcun1D5 Activators
Dcun1D5 employ various biochemical mechanisms to enhance the activity of this E3 ubiquitin ligase. Phorbol 12-myristate 13-acetate (PMA) activates Protein Kinase C (PKC), which can lead to the phosphorylation of Dcun1D5, thereby enhancing its ubiquitin ligase activity. Forskolin raises intracellular cyclic AMP (cAMP) levels, activating Protein Kinase A (PKA), which is another kinase capable of phosphorylating Dcun1D5. Similarly, 8-Bromo-cAMP, a cAMP analog, activates PKA, which may also phosphorylate Dcun1D5. The phosphorylation state of a protein like Dcun1D5 often influences its activity, stability, and interaction with substrates and regulatory proteins, thereby modulating its role in ubiquitination processes within the cell.
Additional regulators include Ionomycin, which is a calcium ionophore that heightens intracellular calcium levels, and this in turn can activate calcium-dependent kinases with the capacity to phosphorylate Dcun1D5. Okadaic Acid and Calyculin A work by inhibiting protein phosphatases such as PP1 and PP2A, which prevents dephosphorylation of proteins, keeping proteins like Dcun1D5 in a phosphorylated and active state. Pervanadate, as an inhibitor of protein tyrosine phosphatases, ensures the phosphorylation of tyrosine residues on proteins, which might affect Dcun1D5 activity. Anisomycin, while inhibiting protein synthesis, can also activate stress-activated protein kinases that may target Dcun1D5 or associated proteins for phosphorylation, thus modulating Dcun1D5 activity. These chemical activators, through their distinct interactions with cellular signaling pathways, contribute to the regulation and maintenance of Dcun1D5's functional state.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA is known to activate Protein Kinase C (PKC), which can phosphorylate target proteins, potentially leading to the activation of downstream proteins, including Dcun1D5. PKC-mediated phosphorylation often results in altered protein-protein interactions and functional consequences, such as increased activity of certain E3 ubiquitin ligases, a category to which Dcun1D5 belongs. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels. Calcium is a secondary messenger that activates calmodulin, which in turn can activate various calcium-dependent kinases. These kinases can phosphorylate a wide spectrum of proteins, potentially including Dcun1D5, thereby enhancing its activity. | ||||||
8-Bromo-cAMP | 76939-46-3 | sc-201564 sc-201564A | 10 mg 50 mg | $126.00 $328.00 | 30 | |
8-Bromo-cAMP is a cell-permeable cAMP analog that activates PKA. Activation of PKA may lead to phosphorylation of proteins that interact with or regulate Dcun1D5, leading to an enhancement of Dcun1D5 ubiquitin ligase activity. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $291.00 $530.00 $1800.00 | 78 | |
Okadaic Acid acts as an inhibitor of protein phosphatases PP1 and PP2A, thus maintaining proteins in a phosphorylated state. This inhibition can result in the sustained activity of kinases and their target proteins. If Dcun1D5 activity is regulated by phosphorylation, okadaic acid would enhance Dcun1D5's function by preventing dephosphorylation. | ||||||
Calyculin A | 101932-71-2 | sc-24000 sc-24000A | 10 µg 100 µg | $163.00 $800.00 | 59 | |
Similar to Okadaic Acid, Calyculin A inhibits protein phosphatases such as PP1 and PP2A. By inhibiting these phosphatases, phosphorylation on various proteins is maintained, which could include Dcun1D5 or its regulatory proteins, thereby potentially enhancing Dcun1D5's activity. | ||||||
Insulin | 11061-68-0 | sc-29062 sc-29062A sc-29062B | 100 mg 1 g 10 g | $156.00 $1248.00 $12508.00 | 82 | |
Insulin activates the insulin receptor, leading to the activation of the PI3K/Akt signaling pathway, which is involved in the regulation of many proteins through phosphorylation. Akt, a serine/threonine kinase, can phosphorylate and change the activity of a multitude of target proteins, which could include Dcun1D5, enhancing its E3 ubiquitin ligase activity. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $99.00 $259.00 | 36 | |
Anisomycin is a protein synthesis inhibitor that also activates stress-activated protein kinases such as JNK and p38 MAP kinase. Activation of these kinases can lead to the phosphorylation of a variety of substrates that might include Dcun1D5 or its associated proteins, potentially leading to an increase in Dcun1D5's ubiquitin ligase activity. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
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